Computation between posterior parietal cortex and its higher order thalamic target during multisensory processing and conflict

多感觉处理和冲突期间后顶叶皮层与其高阶丘脑目标之间的计算

• 批准号: 22KJ3082
• 负责人: Eltabbal Mohamed
• 金额: $ 1.09万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22KJ3082/
• 关键词: Corticothalamic; PPC; tongue

My project involves imaging corticothalamic projection from the posterior parietal cortex (PPC) to its higher-order thalamic nucleus during a multisensory behavioral task. I used a specific Cre-mouse to target layer 6 corticothalamic projections. I found that layer 6 corticothalamic makes sub-additive sensory computation when integrating multisensory signals with many cells showing multiplexed sensory and motor information coding. Currently, I am continuing to image, manipulate, and analyze corticothalamic activity during the task. In addition, I developed a novel behavioral task to study discrete tongue kinematics during interception-like behavior. Using pharmacological inactivation experiments I found that the cerebellum and PPC are necessary for successful execution of such behavior. As the new behavioral paradigm requires less training time this will allow me to image the changes across different stages of learning. I am currently using two photon in-vivo imaging and optogenetics to precisely control the timing of inactivation during the behavior task. I submitted one paper as a coauthor where I used my two-photon imaging analysis pipeline and presented my novel behavioral paradigm in one of the conferences.

我的项目涉及在多感觉行为任务中从后顶叶皮层（PPC）到其高阶丘脑核的皮质丘脑投影成像。我用了一种特殊的Cre-mouse来瞄准第六层皮质丘脑的投影。我发现第6层皮质丘脑在整合多感觉信号时进行了次加性的感觉计算，其中许多细胞显示了多路感觉和运动信息编码。目前，我正在继续成像，操作和分析任务期间的皮质丘脑活动。此外，我开发了一个新的行为任务来研究离散舌运动学在类拦截行为。通过药理学灭活实验，我发现小脑和PPC对于成功执行这种行为是必要的。由于新的行为范式需要更少的训练时间，这将使我能够想象不同学习阶段的变化。我目前正在使用双光子体内成像和光遗传学来精确控制行为任务中失活的时间。我以合著者的身份提交了一篇论文，其中我使用了我的双光子成像分析管道，并在一次会议上展示了我的新行为范式。

项目成果

Grasping the tongue: Cerebeller control of adaptive tongue kinematics

抓住舌头：自适应舌头运动学的小脑控制

• 发表时间: 2022
• 作者: Kosaka Naoko;Kumano Yoshisuke;Kosaka Naoko;Kosaka Naoko;小坂 那緒子;小坂 那緒子;Mohamed EL Tabbal
• 通讯作者: Mohamed EL Tabbal