Microbial synthesis of polyhydroxyalkanoate bearing phenyl and phenylalkyl side groups

点击翻译按钮获取中文摘要

A novel type of 3-hydroxybutyrate (3HB)-based polyhydroxyalkanoate (PHA) copolymer, poly(3HB-co-3-hydroxy-3-phenylpropioate) [P(3HB-co-3H3PhP)], which bears phenyl side group, was synthesized by employing Ralstonia eutropha PHB-4 recombinant expressing Pseudomonas sp. 61-3 PHA synthase 1. The 3H3PhP fraction was increased up to 8.9 mol% by feeding 3H3PhP precursors. As phenyl side group was introduced into P(3HB), the melting temperature and the enthalpy of fusion were decreased while the glass transition temperature was increased, demonstrating a distinct thermal behavior by the effect of phenyl side group.