Direct reprogramming of fibroblasts to hemetopoietic stem cells

我们已经做了以下实验：（1）我们产生了SCL-报告小鼠品系，并使用来自这些小鼠品系的成纤维细胞筛选了“候选基因”。(2)尽管某些“候选基因”组合的转导似乎在一小群转导细胞中诱导EGFP表达，但这些细胞不能在受体小鼠中重建造血。(3)我们通过Crispr产生了在c-kit基因座中携带突变的突变小鼠品系，并检查了（2）中描述的细胞的造血重建能力。这些细胞不能在受体小鼠中重建造血，因此，我们得出结论，我们的“候选基因”不能将成纤维细胞重编程为造血干细胞。

We have done following experiments; (1) We generated SCL-reporter mouse strains and have screened "candidates genes" using fibroblasts derived from these mouse strains. (2) Although the transduction of a certain combination of "candidates genes" seemed to induce EGFP expression in a small population of transduced cells, these cells could not reconstitute hematopoiesis in recipient mice. (3) We have generated the mutant mouse strains harboring the mutations in the c-kit locus by Crispr and examined the hematopoietic reconstitution ability of the cells described in (2). These cells could not reconstitute hematopoiesis in recipient mice.Thus, we have concluded that our "candidates genes" could not reprogram fibroblasts to hematopoietic stem cells.