Direct reprogramming of fibroblasts to hemetopoietic stem cells

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We have done following experiments; (1) We generated SCL-reporter mouse strains and have screened "candidates genes" using fibroblasts derived from these mouse strains. (2) Although the transduction of a certain combination of "candidates genes" seemed to induce EGFP expression in a small population of transduced cells, these cells could not reconstitute hematopoiesis in recipient mice. (3) We have generated the mutant mouse strains harboring the mutations in the c-kit locus by Crispr and examined the hematopoietic reconstitution ability of the cells described in (2). These cells could not reconstitute hematopoiesis in recipient mice.Thus, we have concluded that our "candidates genes" could not reprogram fibroblasts to hematopoietic stem cells.