Direct reprogramming of fibroblasts to hemetopoietic stem cells

成纤维细胞直接重编程为造血干细胞

• 批准号: 23659202
• 负责人: ITOH Katsuhiko
• 金额: $ 2.5万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23659202/
• 关键词: 再生医学; 造血幹細胞検出法; リプログラミング; 造血幹細胞

We have done following experiments; (1) We generated SCL-reporter mouse strains and have screened "candidates genes" using fibroblasts derived from these mouse strains. (2) Although the transduction of a certain combination of "candidates genes" seemed to induce EGFP expression in a small population of transduced cells, these cells could not reconstitute hematopoiesis in recipient mice. (3) We have generated the mutant mouse strains harboring the mutations in the c-kit locus by Crispr and examined the hematopoietic reconstitution ability of the cells described in (2). These cells could not reconstitute hematopoiesis in recipient mice.Thus, we have concluded that our "candidates genes" could not reprogram fibroblasts to hematopoietic stem cells.

我们已经做了以下实验：（1）我们产生了SCL-报告小鼠品系，并使用来自这些小鼠品系的成纤维细胞筛选了“候选基因”。(2)尽管某些“候选基因”组合的转导似乎在一小群转导细胞中诱导EGFP表达，但这些细胞不能在受体小鼠中重建造血。(3)我们通过Crispr产生了在c-kit基因座中携带突变的突变小鼠品系，并检查了（2）中描述的细胞的造血重建能力。这些细胞不能在受体小鼠中重建造血，因此，我们得出结论，我们的“候选基因”不能将成纤维细胞重编程为造血干细胞。

项目成果

Identification of a novel enhancer that binds Sp1 and contributes to induction of cold-inducible RNA-binding protein (cirp) expression in mammalian cells.

• DOI: 10.1186/1472-6750-12-72
• 发表时间: 2012-10-10
• 期刊: BMC biotechnology
• 影响因子: 3.5
• 作者: Sumitomo Y;Higashitsuji H;Higashitsuji H;Liu Y;Fujita T;Sakurai T;Candeias MM;Itoh K;Chiba T;Fujita J
• 通讯作者: Fujita J

Identification of IL-7-Producing Cells in Primary and Secondary Lymphoid Organs Using IL-7-GFP Knock-In Mice

• DOI: 10.4049/jimmunol.1200586
• 发表时间: 2012-08-15
• 期刊: JOURNAL OF IMMUNOLOGY
• 影响因子: 4.4
• 作者: Hara, Takahiro;Shitara, Soichiro;Ikuta, Koichi
• 通讯作者: Ikuta, Koichi

Epigenetic Regulation of Mouse Sex Determination by the Histone Demethylase Jmjd1a

• DOI: 10.1126/science.1239864
• 发表时间: 2013-09-06
• 期刊: SCIENCE
• 影响因子: 56.9
• 作者: Kuroki, Shunsuke;Matoba, Shogo;Tachibana, Makoto
• 通讯作者: Tachibana, Makoto

Light-induced silencing of neural activity in Rosa26 knock-in mice conditionally expressing the microbial halorhodopsin eNpHR2.0

光诱导条件性表达微生物盐视紫红质 eNpHR2.0 的 Rosa26 敲入小鼠神经活动沉默

• 发表时间: 2012
• 期刊: Neurosci. Res
• 作者: Imayoshi;I.;Tabuchi;S.;Hirano;K.;Sakamoto;M.;Kitano;S.;Miyachi;H.;Yamanaka;A.;and Kageyama;R
• 通讯作者: R

HTLV-I Tax regulates the cellular proliferation through the down-regulation of PIP3-phosphatase expressions via the NK-kB pathway.

HTLV-I Tax 通过 NK-kB 途径下调 PIP3 磷酸酶表达来调节细胞增殖。

• 发表时间: 2012
• 期刊: Int. J. Biochem. Mol. Biol.
• 作者: Fukuda RI;Tsuchiya K;Suzuki K;Itoh K;Fujita J;Utsunomiya A and Tsuji T
• 通讯作者: Utsunomiya A and Tsuji T