Development of a direct transfection method in Plasmodium falciparum

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At present, genetic manipulation of Plasmodium falciparum, the most important human malaria parasite is carried out with indirect transfection method that is of low efficiencies. In this study we attempted to develop a direct transfection method in this parasite andimprove the efficiencies. By establishing a method to purify a large amount of mature schizonts and using a P. falciparum artificial chromosome, we succeeded in introducing a foreign DNA into the parasites with approximately 1000-fold efficiency compared to that with indirect transfection method. The method developed in this study will improve genetic manipulation of this parasite and have a great impact on this research field.