Development of a direct transfection method in Plasmodium falciparum

恶性疟原虫直接转染方法的开发

• 批准号: 23659210
• 负责人: YUDA Masao
• 金额: $ 2.41万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23659210/
• 关键词: 原虫; マラリア; 遺伝子導入

At present, genetic manipulation of Plasmodium falciparum, the most important human malaria parasite is carried out with indirect transfection method that is of low efficiencies. In this study we attempted to develop a direct transfection method in this parasite andimprove the efficiencies. By establishing a method to purify a large amount of mature schizonts and using a P. falciparum artificial chromosome, we succeeded in introducing a foreign DNA into the parasites with approximately 1000-fold efficiency compared to that with indirect transfection method. The method developed in this study will improve genetic manipulation of this parasite and have a great impact on this research field.

恶性疟原虫是人类最重要的疟原虫之一，目前对恶性疟原虫的基因操作主要采用间接转染法，效率较低。本研究试图建立一种直接转染法，提高转染效率。通过建立一种纯化大量成熟疟原虫的方法，并使用恶性疟原虫人工染色体，我们成功地将外源DNA导入疟原虫，与间接转染方法相比，效率约为1000倍。本研究开发的方法将提高该寄生虫的遗传操作，并对该研究领域产生重大影响。

项目成果

Prevention of Experimental Cerebral Malaria by Flt3 Ligand during Infection with Plasmodium berghei ANKA

• DOI: 10.1128/iai.01337-10
• 发表时间: 2011-10-01
• 期刊: INFECTION AND IMMUNITY
• 影响因子: 3.1
• 作者: Tamura, Takahiko;Kimura, Kazumi;Yui, Katsuyuki
• 通讯作者: Yui, Katsuyuki

a Transcription Factor That Is Critical for Malaria Liver Stage Development

对疟疾肝脏阶段发育至关重要的转录因子

• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Iwanaga;S.;T. Kato;I. Kaneko & M. Yuda
• 通讯作者: I. Kaneko & M. Yuda

Liver-specific protein 2: a Plasmodium protein exported to the hepatocyte cytoplasm and required for merozoite formation

• DOI: 10.1111/mmi.12083
• 发表时间: 2013-01-01
• 期刊: MOLECULAR MICROBIOLOGY
• 影响因子: 3.6
• 作者: Orito, Yuki;Ishino, Tomoko;Yuda, Masao
• 通讯作者: Yuda, Masao

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Altered gene and protein expression in liver of the obese spontaneously hypertensive/NDmcr-cp rat.

• DOI: 10.1186/1743-7075-9-87
• 发表时间: 2012-09-21
• 期刊: Nutrition & metabolism
• 影响因子: 4.5
• 作者: Chang J;Oikawa S;Ichihara G;Nanpei Y;Hotta Y;Yamada Y;Tada-Oikawa S;Iwahashi H;Kitagawa E;Takeuchi I;Yuda M;Ichihara S
• 通讯作者: Ichihara S