Exploration of malaria parasite genes involved in host-invasion using a transcription factor as a clue

以转录因子为线索探索参与宿主入侵的疟原虫基因

• 批准号: 20249023
• 负责人: YUDA Masao
• 金额: $ 30.62万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20249023/
• 关键词: マラリア; 転写因子; ゲノム; 感染機構; マラリア原虫; ライフサイクル

Malaria parasites have three host-invasive stages in the life cycle. The ookinete is one of these stages, in which malaria parasites invade the midgut of the mosquito. The purpose of this study was to identify target genes of AP2-O and elucidate roles of these genes in ookinetes. By genome-wide analysis with ChIP-Seq, over two thousand of binding sites of AP2-O were identified in the genome of the rodent malaria parasite, Plasmodium. berghei. Based on this result, over four hundred of target genes, which contained all genes that had been reported to be specifically expressed in the ookinete, were identified. Targeted gene disruption experiments in these genes revealed that they contained not only genes for midgut-invasion, but also those necessary for ookinete formation and those prepared in ookinetes and then used after mid-gut invasion. These results indicated that Ap2-O acts as a master transcription factor in the ookinete stage.

疟疾寄生虫在生命周期中有三个宿主侵入阶段。动合子是这些阶段中的一个，在此阶段，疟原虫侵入蚊子的中肠。本研究的目的是鉴定AP 2-O的靶基因，并阐明这些基因在动合子中的作用。通过使用ChIP-Seq的全基因组分析，在啮齿动物疟疾寄生虫疟原虫的基因组中鉴定出超过2000个AP 2-O结合位点。伯吉。在此基础上，鉴定了400多个靶基因，其中包括所有已报道的在动合子中特异表达的基因。这些基因的靶向基因破坏实验表明，它们不仅含有中肠入侵的基因，而且还含有动合子形成所必需的基因，以及在动合子中制备并在中肠入侵后使用的基因。这些结果表明Ap 2-O在动合子阶段起着主转录因子的作用。

项目成果

LISP1 is important for the egress of Plasmodium berghei parasites from liver cells

• DOI: 10.1111/j.1462-5822.2009.01333.x
• 发表时间: 2009-09-01
• 期刊: CELLULAR MICROBIOLOGY
• 影响因子: 3.4
• 作者: Ishino, Tomoko;Boisson, Bertrand;Baldacci, Patricia
• 通讯作者: Baldacci, Patricia

Functional Identification of the Plasmodium Centromere and Generation of a Plasmodium Artificial Chromosome

• DOI: 10.1016/j.chom.2010.02.010
• 发表时间: 2010-03-18
• 期刊: CELL HOST & MICROBE
• 影响因子: 30.3
• 作者: Iwanaga, Shiroh;Khan, Shahid M.;Waters, Andrew P.
• 通讯作者: Waters, Andrew P.

マラリア原虫人工染色体

疟原虫人工染色体

• 发表时间: 2009

Identification of Transcription factor of Malaria Sporozoite.

疟疾子孢子转录因子的鉴定。

• 发表时间: 2010
• 作者: S.Iwanaga;I.Kaneko;M.Yuda
• 通讯作者: M.Yuda

Functional Characterization of the plasmodium centromere and generation of a plasmodium artificial chromosome.

疟原虫着丝粒的功能表征和疟原虫人工染色体的生成。

• 发表时间: 2009
• 作者: S.Iwanaga;M.Yuda;I.Kaneko;C.J.Janse;A.P.Waters Washington
• 通讯作者: A.P.Waters Washington