Photodynamic diagnosis and treatment for local multiple-drug-resistant bacterial infection using photosensitizers that preferably accumulate into bacteria.

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With an established murine intractable MRSA arthritis model using bioluminescent MRSA and resin microparticle, we examined both therapeutic and preventive effect of PDT using methylene blue. Although bioluminescent intensity of MRSA was not diminished immediately after PDT, it was gradually decreased for 6 days after PDT. The therapeutic effect was lost by intravenous administration of anti-neutrophil antibody. Moreover, pre-conditioning PDT on the normal knee joint prevented an exacerbation of MRSA infection of the knee joint. The preventive effect was also lost by intravenous administration of anti-neutrophil antibody. On the other hand, an adjuvant use of antibiotics for MRSA either linezolid or vancomycin exerted no enhancing effect of MB-PDT, suggesting that they might have inhibited the PDT induced accumulating effect of neutrophils into infected lesions.