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Aquaporin 1 is involved in the adhesion and migration of mesenchymal tissue stem cells/cartilage progenitor cells in the human ear perichondrium

水通道蛋白1参与人耳软骨膜间充质组织干细胞/软骨祖细胞的粘附和迁移

基本信息

批准号：
23659839
负责人：
HIMI Yuji
金额：
$ 2.25万
依托单位：
Kanazawa Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2012
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23659839/
关键词：
アクアポリン1 軟骨膜細胞アクアポリン１ AQP1 軟骨再生

项目摘要

Aquaporin 1 was expressed in the perichondrial cells, both cultured and within the ear perichondrium tissue. Inhibition of aquaporin 1 expression by siRNA did not affect cell proliferation or differentiation to chondrocytes, but significantly reduced both the adhesion to type I collagen and migration of the two- dimensional cultured perichondrial cells. Cartilage tissue formation in the three-dimensional culture was significantly inhibited by RNA interference for aquaporin 1 gene. These data verified the mesenchymal tissue stem cell features of the ear perichondrial cells and the involvement of aquaporin1 in both their adhesion to extracellular matrix and migration. Aquaporin 1 may be apromising molecule for establishing a novel regenerative medicine for craniofacial elastic cartilage.

水通道蛋白1在培养的和耳软骨膜组织内的软骨膜细胞中表达。通过siRNA抑制水通道蛋白1表达不影响细胞增殖或向软骨细胞的分化，但显著降低了二维培养的软骨膜细胞对I型胶原的粘附和迁移。水通道蛋白1基因的RNA干扰显著抑制了软骨组织的形成。这些数据证实了间充质组织干细胞的特性，耳软骨膜细胞和水通道蛋白1的参与，他们的粘附细胞外基质和迁移。水通道蛋白1可能是一个很有前途的分子，为建立一个新的再生医学颅面弹性软骨。