Bone modeling system in medaka

青鳉骨骼建模系统

• 批准号: 23659856
• 负责人: KUDOU Akira
• 金额: $ 2.33万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23659856/
• 关键词: 口腔解剖学(含組織学発生学); メダカ; 破骨細胞; RANKL; NFATc1; 発生・分化; 細胞･組織; 遺伝子; トランスジェニック; 骨形成; リモデリング

To investigate the mechanism of bone modeling in pharyngeal teeth and vertebral bone of medaka, we have developed the osteoblast and osteoclast specific double transgenic medaka which visualizes osteoblasts as a red fluorescence and osteoclasts as a green fluorescence in the live imaging. In the results, osteoclasts trace the trail of osteoblasts to expand the osteoclast area on neural arch. In the cell fusion of osteoclasts, cell-cell interaction of two osteoclasts on the neural arch is required. In the new established fracture model by using caudal bone, two different osteoclasts act for bone modeling and remodeling. In the analysis of RANKL knock-out medaka, osteoclasts are developed and generated in the RNAKL independent manner.

为了研究青鳉咽齿和脊椎骨的造骨机制，我们研制了成骨细胞和破骨细胞特异性双转基因青鳉，在活体成像中，成骨细胞显示为红色荧光，破骨细胞显示为绿色荧光。实验结果表明，破骨细胞沿着成骨细胞的轨迹，扩大了破骨细胞在神经弓上的面积，破骨细胞的细胞融合需要神经弓上两个破骨细胞之间的细胞-细胞相互作用。在新建立的尾状骨骨折模型中，两种不同的破骨细胞起着骨造模和骨重建的作用。在RANKL敲除青鳉的分析中，破骨细胞以RNAKL独立的方式发育和产生。

项目成果

The rankl knock-out medaka exhibits a defective phenotype of bone resorption following abnormal organogenesis together with a small number of osteoclasts regulated by RANKL-independent osteoclasogenesis

rankl 敲除青鳉在器官发生异常后表现出骨吸收缺陷表型以及少量由 RANKL 独立的破骨细胞生成调节的破骨细胞

• 发表时间: 2013
• 作者: Chatani;M.
• 通讯作者: M.

K. In vivo imaging of medaka fracture healing reveal that cyclooxygenase-2 plays an important role in inducing osteoclasts for bone remodeling

K.青鳉骨折愈合的体内成像揭示环氧合酶-2在诱导破骨细胞进行骨重塑方面发挥重要作用

• 发表时间: 2012
• 作者: Takeyama;K
• 通讯作者: K

The rankl knock-out medaka exhibits a defective phenotype of bone resorption following abnormal organogenesis together with a small number of osteoclasts regulated by rankl-independent osteoclastogenesis

Rankl 敲除青鳉在器官发生异常后表现出骨吸收缺陷表型以及少量由独立于Rankl的破骨细胞生成调节的破骨细胞

• 发表时间: 2013
• 作者: 村上愛;曽我康一;小竹敬久;加藤壮英;橋本隆;若林和幸;保尊隆享;Masahiro Chatani
• 通讯作者: Masahiro Chatani

In vivo imaging of medaka fracture healing reveal that cyclooxigenase-2 plays an important role in inducing osteoclasts for bone remodeling

青鳉骨折愈合的体内成像揭示环加氧酶-2在诱导破骨细胞进行骨重塑方面发挥着重要作用

• 发表时间: 2012
• 作者: Takeyama;K.
• 通讯作者: K.

加重力環境下における破骨細胞活性化のメカニズム

重力环境下破骨细胞激活机制

• 发表时间: 2012
• 作者: Chatani;M.;萬徳晃子
• 通讯作者: 萬徳晃子