Bone modeling system in medaka

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To investigate the mechanism of bone modeling in pharyngeal teeth and vertebral bone of medaka, we have developed the osteoblast and osteoclast specific double transgenic medaka which visualizes osteoblasts as a red fluorescence and osteoclasts as a green fluorescence in the live imaging. In the results, osteoclasts trace the trail of osteoblasts to expand the osteoclast area on neural arch. In the cell fusion of osteoclasts, cell-cell interaction of two osteoclasts on the neural arch is required. In the new established fracture model by using caudal bone, two different osteoclasts act for bone modeling and remodeling. In the analysis of RANKL knock-out medaka, osteoclasts are developed and generated in the RNAKL independent manner.