Diagnostic bioimaging for acute cellular rejection using antibody probes in mouse liver transplantation

在小鼠肝移植中使用抗体探针对急性细胞排斥进行诊断生物成像

• 批准号: 23659607
• 负责人: MITSUCHIKA Hosoda
• 金额: $ 2.33万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23659607/
• 关键词: 肝移植; 拒絶反応; 生体イメージング

We conducted this study to develop a new diagnostic method for allograft rejection in liver transplantation where infiltrating T-cells were quantified using antibody probes. We succeeded in inducing an acute cellular rejection in a mouse model of allogeneic liver transplantation. Immunohistologically, we confirmed that CD3 was strongly expressed on the infiltrating T-cells. However, a biological imaging after systemic injection of anti CD3 antibody probes could not detect the infiltrating T-cells. To deliver antibody probes to the infiltrating T-cells directly, we developed an extracorporeal perfusion machine for mouse liver and administered antibody probes to liver graft via the perfusion fluid. Even in this method, anti CD3 antibody probes could not detect the infiltrating T-cells.

我们进行这项研究是为了开发一种新的诊断肝移植排斥反应的方法，其中浸润t细胞使用抗体探针进行量化。我们成功地在小鼠同种异体肝移植模型中诱导急性细胞排斥反应。免疫组织学上，我们证实CD3在浸润的t细胞上强烈表达。然而，全身注射抗CD3抗体探针后的生物成像无法检测到浸润的t细胞。为了将抗体探针直接输送到浸润的t细胞中，我们研制了一种小鼠肝脏体外灌注机，并通过灌注液将抗体探针注入移植物中。即使在这种方法中，抗CD3抗体探针也不能检测到浸润的t细胞。