Developmental transcriptome analysis of monkey brain for the establishment of human disease model

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For the establishment of primate model of human neuropsychiatric diseases and high-level cognitive functions, we performed spatio-temporal brain transcriptome analysis of macaques covering 12 brain regions and 7 developmental stages. High throughput transcriptome analysis by next generation sequencer reveals that profile of gene expression is more similar between brain regions within individuals than between individuals in the same brain regions.We also aimed to elucidate the molecular basis of regional and circuit specificities at the single neuron level. To execute this, three different fluorescent retrograde tracers were injected into three distinct part of monkey brains (thalamus, striatum, pons), and three distinct signals on neurons were successfully confirmed in the prefrontal regions. At this moment, we are going to profile molecular signatures of regional and circuit specific neurons by collecting each neuron based on the fluorescent signal by laser capture microdissection.