Direct manipulation of the intracellular stress distribution for controlling cell division

在这项研究中，我们开发了一种技术来测量细胞拉伸过程中细胞内应力分布的动态变化，我们也建立了一种控制细胞内张力的技术。利用这些技术，我们研究了机械刺激（如细胞拉伸）对HeLa细胞分裂和增殖的影响。我们发现，在有丝分裂过程中，细胞纺锤体方向在垂直于细胞赤道平面的拉伸后发生了巨大的变化：HeLa细胞沿垂直于拉伸方向分裂。这些结果表明，细胞分裂是根据施加在细胞上的力所提供的机械因素。我们的技术将有助于研究细胞分裂的机械转导机制。

In this study, we developed a technique to measure the dynamic changes in intracellular stress distribution during cell stretching, and we also established to manipulate intracellular tension. Using these techniques, we investigated the effects of mechanical stimuli, such as cell stretching, on the division and proliferation of HeLa cells. We found that the cell spindle orientation during mitosis dramatically changed following cell stretching in perpendicular to the equator plane of the cells: HeLa cells divided in perpendicular to the stretch direction. These results indicate that cells divide according to mechanical factors provided by the forces applied to the cells. Our technique would be useful in investigating the mechanotransduction mechanisms of dividing cells.