Control of cell functions by mechanical stimulation of cell nuclei

通过细胞核的机械刺激控制细胞功能

• 批准号: 22650104
• 负责人: NAGAYAMA Kazuaki
• 金额: $ 2.11万
• 依托单位: Nagoya Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22650104/
• 关键词: バイオメカニクス; 生物・生体工学; メカノトランスダクション; 細胞骨格; 細胞分裂; 微細加工技術; 力学特性; 細胞の力学応答

The purpose of this study is to elucidate the changes in the mechanical environment of cell nuclei during cell differentiation, and to establish a method to apply mechanical stimuli to intracellular nucleus directly. We found that actin cytoskeletal filaments have crucial roles in the intracellular force balance three dimensionally during cell differentiation, which control the three dimensional morphology of nucleus. We also developed a magnetic micropillar array substrate made of silicone elastomer(poly(dimethylsiloxane): PDMS). We successfully embedded iron particles into the micropillars and controlled their location in the substrate. Application of external magnetic field creates forces on the particles in the pillars, and an external force can be transferred to nuclei of cells. This technique can be utilized for direct mechanical stimulation of cell nuclei.

本研究旨在阐明细胞分化过程中细胞核力学环境的变化，并建立一种直接对细胞内核施加力学刺激的方法。我们发现，肌动蛋白细胞骨架丝在细胞分化过程中的三维力平衡中起着至关重要的作用，它控制着细胞核的三维形态。我们还开发了一种磁性微柱阵列基板的硅橡胶（聚（二甲基硅氧烷）：PDMS）。我们成功地将铁颗粒嵌入微柱中并控制它们在基底中的位置。施加外部磁场在柱中的颗粒上产生力，并且外力可以被传递到细胞核。该技术可用于细胞核的直接机械刺激。

项目成果

On the roles of actin stress fibers on mechanical stabilization of the nucleus and intracellular DNA

肌动蛋白应力纤维对细胞核和细胞内 DNA 机械稳定的作用

• 发表时间: 2011
• 作者: Tomikawa M;Hong J;Shiotani S;Tokunaga E;Konishi K;Ieiri S;Tanoue K;Akahoshi T;Maehara Y;Hashizume M;Nagayama K
• 通讯作者: Nagayama K

Dynamic Changes of Traction Force at Focal Adhesions during Macroscopic Cell Stretching Using an Elastic Micropillar Substrate: Tensional Homeostasis of Aortic Smooth Muscle Cells

使用弹性微柱基底宏观细胞拉伸过程中局部粘连牵引力的动态变化：主动脉平滑肌细胞的张力稳态

• DOI: 10.1299/jbse.7.130
• 发表时间: 2012
• 期刊: Journal of Biomechanical Science and Engineering
• 作者: Nagayama;K.;Adachi;A.;Matsumoto;T.
• 通讯作者: T.

Heartbeat regulates cardiogenesis by suppressing retinoic acid signaling via expression of miR-143

• DOI: 10.1016/j.mod.2010.09.002
• 发表时间: 2011-02
• 期刊: Mechanisms of Development
• 影响因子: 2.6
• 作者: K. Miyasaka;Yasuyuki S. Kida;Toshihiro Banjo;Y. Ueki;K. Nagayama;Takeo Matsumoto;Masaaki Sato;T. Ogura

細胞の力学応答解析ツールとしての磁気駆動式マイクロピラーデバイスの開発

开发磁驱动微柱装置作为分析细胞机械响应的工具

• 发表时间: 2011
• 作者: 高倉久志;古市泰郎;山田達也;岩中伸壮;岩瀬敏;増田和実;長谷川博;Tsuyoshi Kimura;長山和亮
• 通讯作者: 長山和亮

Estimation of Single Stress Fiber Stiffness in Cultured Aortic Smooth Muscle cells under Relaxed and Contracted States

培养的主动脉平滑肌细胞在松弛和收缩状态下单应力纤维刚度的估计

• 发表时间: 2010
• 期刊: J Biomech
• 影响因子: 2.4
• 作者: Nakamura T;Phuong THN;Hori E;Urakawa S;Uwano T;Hamashima T;Ishii Y;Matsushima T;Ono T;Sasahara M;Nishijo H.;Matsumoto T;佐々木えりか;Nagayama K
• 通讯作者: Nagayama K