Manipulation of high-density lipoprotein structure and function for development of gene delivery carrier

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Our purpose in this study is to develop nanomaterials capable of delivering therapeutic gene to desired sites, such as cancer tissues, by utilizing natural nanomaterials in our body. High-density lipoprotein (HDL) is a natural nanomaterial that delivers lipids from peripheral tissues to the liver in our body. Due to its physiological functions, HDL have a propensity to accumulate in liver via HDL receptor recognition of apoA-I moiety in HDL. In order to weaken this recognition in the liver and then to redirect HDL to desired sites, we designed 9 apoA-I deletion mutants and succeeded in preparation of 8 HDL mutants. Among them, a few HDL mutants were found to show decreased affinity to the HDL receptor.