Biomedical engineering approach to establish a new evaluation system for osteoconductivity by focusing on the protein adsorption properties to biomaterials

生物医学工程方法通过关注生物材料的蛋白质吸附特性建立新的骨传导性评价体系

• 批准号: 24650272
• 负责人: KANETAKA Hiroyasu
• 金额: $ 2.41万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650272/
• 关键词: バイオマテリアル; 骨伝導能; 医工学

The aim of this study was to establish a new evaluation system for osteoconductivity by focusing on the protein adsorption properties to biomaterials. First, we revealed the difference in the three-dimensional conformational status of the protein on the surface of such osteoconductive biomaterials as hydroxyapatite, as well as the amount of adsorption, concerning the adsorption behavior of serum proteins. Next, by revealing the correlation between the proliferative capacity of mouse pre-osteoblastic cell line MC3T3-E1 and protein adsorption properties on the material, the possibility of a new evaluation system for osteoconductivity was investigated.

本研究旨在通过研究蛋白质对生物材料的吸附特性，建立一种新的骨导电性评价体系。首先，我们揭示了蛋白质在羟基磷灰石等导骨生物材料表面的三维构象状态的差异，以及吸附量的差异，这与血清蛋白的吸附行为有关。接下来，通过揭示小鼠成骨前细胞系MC3T3-E1的增殖能力与材料上蛋白质吸附特性之间的相关性，探讨一种新的骨导电性评价体系的可能性。

项目成果

Zeta potentials and bovine serum albumin adsorption of -alumina-based ceramic particles

氧化铝基陶瓷颗粒的 Zeta 电位和牛血清白蛋白吸附

• 发表时间: 2013
• 作者: Kawashita M;Hayashi J;Miyazaki T;Hashimoto M;Hihara H;Kanetaka H
• 通讯作者: Kanetaka H

δ型アルミナのアルブミン吸着挙動

δ型氧化铝对白蛋白的吸附行为

• 发表时间: 2012
• 作者: Kawashita M;Hayashi J;Miyazaki T;Hashimoto M;Hihara H;Kanetaka H;林純平,川下将一,宮崎敏樹,橋本雅美,金高弘恭
• 通讯作者: 林純平,川下将一,宮崎敏樹,橋本雅美,金高弘恭

アルブミンを吸着させた水酸アパタイトおよびα型Al2O3に対するMC3T3-E1およびRAW264.7細胞の応答

MC3T3-E1和RAW264.7细胞对白蛋白吸附的羟基磷灰石和α型Al2O3的反应

• 发表时间: 2013
• 作者: Kawashita M;Hayashi J;Miyazaki T;Hashimoto M;Hihara H;Kanetaka H;川下将一,林純平,工藤忠明,金高弘恭,李志霞,宮崎敏樹,橋本雅美
• 通讯作者: 川下将一,林純平,工藤忠明,金高弘恭,李志霞,宮崎敏樹,橋本雅美

Adsorption characteristics of bovine serum albumin on alumina particles with specific crystalline structure

特定晶体结构氧化铝颗粒对牛血清白蛋白的吸附特性

• 发表时间: 2013
• 作者: Kawashita M;Hayashi J;Li;Z Miyazaki T;Hashimoto M;Hihara H;Kanetaka H
• 通讯作者: Kanetaka H

Adsorption characteristics of bovine serum albumin onto alumina with a specific crystalline structure

• DOI: 10.1007/s10856-013-5086-z
• 发表时间: 2014-02
• 期刊: Journal of Materials Science: Materials in Medicine
• 作者: M. Kawashita;Junpei Hayashi;Zhixia Li;T. Miyazaki;M. Hashimoto;Hiroki Hihara;H. Kanetaka