Physiological functions of free branched-chain amino acids in nerve and brain

支链α -酮酸脱氢酶激酶（BDK）抑制支链氨基酸代谢。在本研究中，我们制备了条件bdk敲除（KO）小鼠，以阐明BCAAs的生理功能。我们首先用BDK-floxed小鼠制备条件BDK-KO小鼠。利用小鼠，我们先制备了全身（全身）BDK-KO小鼠，然后是肌肉特异性BDK-KO小鼠，现在正在进行神经特异性BDK-KO小鼠的制备。条件BDK-KO小鼠具有组织特异性BCAAs缺陷。这些老鼠的具体特征正在逐渐被阐明。

Branched-chain amino acid metabolism is suppressed by branched-chain alpha-ketoacid dehydrogenase kinase (BDK). In the present study, we have prepared conditional BDK-knockout (KO) mice to clarify the physiological functions of BCAAs. We first produced BDK-floxed mice for preparation of conditional BDK-KO mice. Using the mice, we prepared global (whole body) BDK-KO mice, and then muscle-specific BDK-KO mice, and now preparation of nerve-specific BDK-KO mice is ongoing. The conditional BDK-KO mice have a tissue-specific deficiency of BCAAs. Specific features of these mice are becoming to be clarified.