Electron micrograph imaging of a single protein without staining

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The object of this research is an imaging of a single protein molecule by a scanning transmission electron microscope (STEM) without staining. The key technology of this research is using a graphene oxide (GO) film as a substrate for STEM observation. The target resolution is 1 nm in the level of lysozyme protein. The outcome of the project were1) Development of a single layer GO film on a Cu microgrid. 2) Preparation of protein sample on a GO single layer under low acceleration voltage without loss of resolution. 3) STEM monitoring of a single NADH ubiquinone reductase whose size is 20 nm without uranyl staining on a GO film.