Estimation of an alternative biosynthetic pathway for co-factors by close investigation of genome databases.

研究了新酶参与辅因子生物合成的情况。pABA;以paba -营养不良大肠杆菌突变体（△pabABC）为寄主，通过鸟枪克隆实验，从欧洲亚硝化单胞菌（Nitrosomonas europaea）中获得了功能未知的基因NE1434。谷胱甘肽;链霉菌株不使用谷胱甘肽。然而，SCO0910被证实是谷氨酸-半胱氨酸连接酶，是麦角硫因生物合成所必需的。氨基乙磺酸;据报道，coelicolor A3(2)中的SCO3035是牛磺酸生物合成中的第一个酶[j] .细菌学报，188,55(6),2006。为了研究S. coelicolor A3(2)、SCO3416、2782和2017中牛磺酸的生物合成，我们使用推定的催化第二反应的酶进行了体外实验。然而，所有的酶都没有表现出预期的活性。

The invovment of new enzymes for co-factors biosynthesis was examined. pABA; A function unknown gene, NE1434, was obtained from Nitrosomonas europaea by shotgun cloning experiment using pABA-auxotrophic E. coli mutant (△pabABC) as a host. Glutathione; Streptomyces strains do not use glutathione. However, SCO0910 was confirmed to be glutamate-cysteine ligase and was essential for ergothioneine biosynthesis. Taurine; SCO3035 in S. coelicolor A3(2) was reported to be the first enzyme in taurine biosynthesis (J. Bacteriol. 188, 5561, 2006). To investigate taurine biosynthesis in S. coelicolor A3(2), SCO3416, 2782, and 2017, putative enzymes catalyzing thesecond reaction, were used for in vitro assay. However, all enzymes did not show the expected activity.