Estimation of an alternative biosynthetic pathway for co-factors by close investigation of genome databases.

研究了用于辅因子生物合成的新酶的发明。PABA；以PABA营养缺陷型大肠杆菌突变体(PabABC)为宿主，通过鸟枪法克隆得到一个功能未知的基因NE1434。链霉菌菌株不使用谷胱甘肽。SCO0910是谷氨酸-半胱氨酸连接酶，对麦角硫氨酸的生物合成是必需的。天蓝色链霉菌(S.coelicolor A3(2))中的SCO3035是合成牛磺酸的第一酶。188、5561、2006)。为了研究天蓝色链霉菌A3(2)中牛磺酸的生物合成，利用推测的催化第二反应的酶SCO3416、2782和2017进行了体外实验。然而，所有的酶都没有表现出预期的活性。

The invovment of new enzymes for co-factors biosynthesis was examined. pABA; A function unknown gene, NE1434, was obtained from Nitrosomonas europaea by shotgun cloning experiment using pABA-auxotrophic E. coli mutant (△pabABC) as a host. Glutathione; Streptomyces strains do not use glutathione. However, SCO0910 was confirmed to be glutamate-cysteine ligase and was essential for ergothioneine biosynthesis. Taurine; SCO3035 in S. coelicolor A3(2) was reported to be the first enzyme in taurine biosynthesis (J. Bacteriol. 188, 5561, 2006). To investigate taurine biosynthesis in S. coelicolor A3(2), SCO3416, 2782, and 2017, putative enzymes catalyzing thesecond reaction, were used for in vitro assay. However, all enzymes did not show the expected activity.