Screening of natural products which inhibits a new menaquinone biosynthetic pathway

呋喃他罗辛途径在一些细菌中运行，用于生物合成孟喹酮。在MqnB作用下，Futalosine被转化为去黄烷基Futalosine(DHFL)。本研究提出了DHFL的三条形成途径。DHFL可能是MqnB在嗜热嗜热菌中直接形成的。在天蓝色链霉菌中，氨基脱氧FL(AFL)被脱氨酶转化为FL，再转化为DHFL。相反，幽门螺杆菌的MqnB直接将AFL转化为DHFL。为了鉴定特异性抑制Futalosin途径的化合物，我们以具有FL途径的嗜盐假单胞菌为测试菌株。最终得到两种候选真菌发酵液。

The futalosine pathway is operating in some bacteria for the biosynthesis of menaquinone. Futalosine is converted into dehypoxanthinyl futalosine (DHFL) by MqnB.In this study, three routes to the formation of DHFL were suggested. DHFL may have been directly formed by MqnB in Thermus thermophilus. In Streptomyces coelicolor, aminodeoxy FL (AFL) was converted to FL by deaminases, then to DHFL. In contrast, MqnB of Helicobacter pylori directly converted AFL into DHFL.To identify compounds that specifically inhibit the futalosin pathway, we used B. halodurans that has FL pathway as a test strain. Finally, two candidate fungal culture broths were obtained.