Folding of cysteine-rich peptides on phospholipid membrane

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In this study, we developed a new protocol for the oxidative folding reaction of cysteine-rich peptides in a phospholipid membrane: "membrane induced folding reaction". We investigated the effect of the phospholipid composition and pH of the reaction on the folding yield and purity of cystein-knot peptides including beta defensins, conotoxins, and other peptides. The best yield was obtained in the presence of glycero-phosholipid membrane with negative charge without adding anr redox reagents. The phospholipid membranes for the folding reaction can be a general method for peptide and protein engineering.