Chemical Biology on the Symbiotic System in Marine Invertebrates

研究表明，海洋次生代谢物大部分来源于共生微生物。本课题以海绵Discodermia花萼中一种独特的海洋代谢物calyculin A为研究对象，对产生calyculin A的微生物进行分离鉴定。我们已经成功地获得了被认为可以产生花青素A的浓缩微生物的部分，并从浓缩微生物的组装中提取了基因组DNA。对提取的基因组DNA进行宏基因组测序分析和后续的DNA定位，使我们能够确定PKS基因簇的主要微生物成分和13个contigs。

It has been disclosed that most of the marine secondary metabolites originate from the symbiotic microbes. In this research project, we focused on an unique marine metabolite, calyculin A from a marine sponge Discodermia calyx, and tried to separate and identify the calyculin A producing microbe. We have succeeded to obtain the fraction with concentrated microbes thought to produce calyculin A and extraction of genomic DNA from this assembly of the concentrated microbes. The metagenomic DNA sequencing analysis of the extracted genomic DNA and the following DNA mapping allowed us to identify the major microbe constituents and 13 contigs of PKS gene clusters.