Molecular-based analysis of organ construction using proteomic analyis

本研究重点研究CTOS膜和细胞外基质的蛋白质表达模式，以期发现与构建三维器官结构相关的分子。此外，我们还创造了将CTOS作为平板培养的新方法。通过生物素化的蛋白质组学分析，发现有几种蛋白质在球状CTOS中特异表达，但在扁平培养的CTOS中不表达，膜和细胞外基质中的表达估计反映了大量培养模式。这些蛋白质可能在构建器官结构中具有重要作用，并将在未来阐明其在三维结构中的活性。

In this study, we focused on protein expression pattern of CTOS membrane and extracellular matrix to discover the molecules related with the construction of 3D organ structure. Moreover, we created new method to culture CTOS as flat culture. Several proteins were identified as specific expression in spheroid CTOS but not flat –cultured CTOS expressed in membrane and extracellular matrix, which estimate to reflect heavily culture patterns, using proteomic analysis with biotinylation. These proteins were suggested to have an important role to construct the organ structure and will be clarify the activity in 3D structure in future.