Creation of functional mutant interferon-alpha for increase of bioactivity and decease of side-effects

本研究旨在利用结构突变体进行干扰素（IFN）治疗的先导性研究，以提高IFN的体内活性，降低其毒副作用。从IFNα8突变体库中筛选出38个与IFN受体结合活性不同的IFNα8突变体。在这些突变体中，我们发现4个突变体IFN活性比野生型IFNα 8高上级。未来，我们将通过分析这些突变体IFN活性提高的机制，利用这些突变体IFN创造新的生物药物。

In this study, we focused on lead discovery for new interferon(IFN) therapy using structural mutant, which increase the activity and decrease the side-effects in vivo. We obtained 38 IFNα8 mutants, which have different binding activities for IFN receptor to perform the screening from IFN mutant library. Among these mutants, we found 4 mutant IFNs have superior activities than wild-type IFNα8.In future, we will utilize these mutant IFNs to create a new biopharmaceuticals by analyzing the mechanisms of increasing activity.