DISTANCE MEASUREMENTS OF FIBROUS PRION PROTEINS BY PULSE ESR SPECTROSCOPY
通过脉冲 ESR 光谱法测量纤维状朊病毒蛋白的距离
基本信息
- 批准号:24654112
- 负责人:
- 金额:$ 2.5万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Challenging Exploratory Research
- 财政年份:2012
- 资助国家:日本
- 起止时间:2012-04-01 至 2014-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This research was performed to clarify the mechanism of formation of the prion filaments. The structure of prion monomer consists of a tertiary C-terminal domain (three alpha-helices, H1, H2 and H3) and an N-terminal random domain. In the experiments, recombinant prion protein with a cysteine mutation was created by E. Coli expression system and the cysteine residue was labeled by nitroxide spin probe. After aggregated prion filaments induced by detergent, the spin-spin interaction was measured by X-band continuous-wave (CW) electron spin resonance (ESR) or Q-band pulse ESR spectrometer. The inter-spin distance was determined by Monte Carlo fitting for electron-electron interaction signals. The inter-spin distance of prion filaments spin-labeled at H1 or H2 is shorter than that of H3. However, the inter-spin distance of N-terminal domain was not estimated because of polydispersity in distance. These data suggested that H1 and H2 were involved in formation of prion filaments.
这项研究是为了阐明Prion丝的形成机制。Prion单体的结构由三个C-末端结构域(三个α-螺旋,H1,H2和H3)和一个N-末端随机结构域组成。本实验利用大肠杆菌表达系统构建了半胱氨酸突变的重组PrP蛋白,并用氮氧化物自旋探针标记了半胱氨酸残基。用X波段连续波(CW)电子自旋共振(ESR)或Q波段脉冲电子自旋共振(ESR)光谱仪测量洗涤剂诱导的聚集性Prion丝的自旋-自旋相互作用。用蒙特卡罗方法对电子-电子相互作用信号进行了拟合,确定了自旋间距。在H1或H2处自旋标记的Prion细丝的自旋间距比H3处的短。然而,由于距离的多分散性,N-末端结构域的自旋间距离无法估计。这些数据表明,H1和H2参与了Prion细丝的形成。
项目成果
期刊论文数量(25)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Redox factor-1 activates endothelial SIRTUIN1 through reduction of conserved cysteine sulfhydryls in its deacetylase domain.
- DOI:10.1371/journal.pone.0065415
- 发表时间:2013
- 期刊:
- 影响因子:3.7
- 作者:Jung SB;Kim CS;Kim YR;Naqvi A;Yamamori T;Kumar S;Kumar A;Irani K
- 通讯作者:Irani K
プリオンタンパク質凝集体の電子スピン共鳴-スピンラベル法による解析
通过电子自旋共振-自旋标记分析朊病毒蛋白聚集体
- DOI:
- 发表时间:2013
- 期刊:
- 影响因子:0
- 作者:稲波修;山盛徹;安井博宣;堀内基広;平岡和佳子;古川貢;中村敏和
- 通讯作者:中村敏和
赤血球膜の不安定性を示すウシαスペクトリンの局所的構造変化に関する研究
牛α-血影蛋白局部结构变化表明红细胞膜不稳定性的研究
- DOI:
- 发表时间:2013
- 期刊:
- 影响因子:0
- 作者:藤本桃子;山盛徹;安井博宣;永根大幹;大津航;木崎皓太;稲葉睦;稲波修
- 通讯作者:稲波修
LONGITUDINAL ANALYSIS OF TUMOR OXYGENATION AFTER THE METABOLIC-TARGETED TREATMENT WITH DICHLOROACETATE USING PULSED ESR IMAGING
使用脉冲 ESR 成像对二氯乙酸代谢靶向治疗后肿瘤氧合进行纵向分析
- DOI:
- 发表时间:2014
- 期刊:
- 影响因子:0
- 作者:Yasui H.;Saito K.;Nishida N.;Matsumoto S.;Yamamori T.;Krishna M.C.;Inanami O
- 通讯作者:Inanami O
電子スピン共鳴法を用いた酸素イメージングによる腫瘍内間欠的低酸素の描出とその意義
电子自旋共振氧成像描绘瘤内间歇性缺氧及其意义
- DOI:
- 发表时间:2013
- 期刊:
- 影响因子:0
- 作者:安井博宣;松元慎吾;齋藤圭太;山盛徹;Murali C. Krishna;稲波修
- 通讯作者:稲波修
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NAKAMURA Toshikazu其他文献
NAKAMURA Toshikazu的其他文献
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{{ truncateString('NAKAMURA Toshikazu', 18)}}的其他基金
Analysis of molecular mechanisms that reciprocally regulate growth and differentiation of mature hepatocytes
成熟肝细胞生长和分化相互调节的分子机制分析
- 批准号:
21390079 - 财政年份:2009
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Investigation of Spin Dynamics and Development of Devices for Low-Dimensional Electronic Phases
自旋动力学研究和低维电子相器件的开发
- 批准号:
20340095 - 财政年份:2008
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular Mechanisms of Tissue Regeneration through the Conversion of HGF Receptor Signaling in Response of Injury
通过损伤反应中 HGF 受体信号转导实现组织再生的分子机制
- 批准号:
18390087 - 财政年份:2006
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular Basis of Anti-fibrosis via Destruction of an Organ Self-repair System
通过破坏器官自我修复系统抗纤维化的分子基础
- 批准号:
14207005 - 财政年份:2002
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Investigation of the electronic states in the successive SDW transitions of one-dimensional organic conductors
一维有机导体连续 SDW 跃迁中电子态的研究
- 批准号:
13640375 - 财政年份:2001
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Studies on Tissue Morphogenesis, Organogenesis and Repair by HGF
HGF 的组织形态发生、器官发生和修复研究
- 批准号:
11308025 - 财政年份:1999
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular and cellular biological analyzes of morphogenesis modulated by HGF
HGF 调节形态发生的分子和细胞生物学分析
- 批准号:
08408027 - 财政年份:1996
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Inhibitory effects of HGF antagonist on invasion/metastasis of tumor cells.
HGF拮抗剂对肿瘤细胞侵袭/转移的抑制作用。
- 批准号:
07557199 - 财政年份:1995
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular mechanisms of organ regeneration and homeoslasis by injurin/HGF system.
Injurin/HGF 系统器官再生和稳态的分子机制。
- 批准号:
05404080 - 财政年份:1993
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)
Development of Large Scale Expression, Preparation, and Highly Sensitive Immunoassay Methods for Hepatocyte Growth Factor
肝细胞生长因子大规模表达、制备和高灵敏免疫分析方法的开发
- 批准号:
03558020 - 财政年份:1991
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)