19F-MR Imaging of Nucleic Acids based on Photochemically Hyperpolarlized Nuclear Spins

体内成像技术，如x射线计算机断层扫描、正电子发射断层扫描和核磁共振成像，是诊断各种疾病和外科治疗后随访的有前途的技术。由于体内19F MR的低背景信号，这些基于19F MR的成像探针具有对目标生物分子进行高对比度和特异性成像的潜力。然而，由于19F磁共振信号的灵敏度较低，用于核酸成像的分子19F探针鲜有报道。利用19F核的副氢诱导超极化（PHIP）可以极大地增强19F核的MR信号。因此，研究了环丁烷二聚体通过DNA交联产生的光化学广义对氢的19F-MR成像。

In vivo imaging technologies, such as X-ray computed tomography, positron emission tomography, and nuclear magnetic resonance imaging, are promising technologies for diagnosis of various disorders and follow up after surgical treatments. Due to the low background signal of 19 F MR in vivo, these 19F MR based imaging probes have potential for high contrast and specific imaging of target biomolecules. However, molecular 19F probes that can image nucleic acids have been scarcely reported, because of low sensitivity of 19F MR signal. As the method that can tremendously enhance the MR signal of 19F nuclei by using of parahydrogen-induced hyperpolarization (PHIP) of 19F nuclei. So, 19F-MR imaging of DNA based on photochemically generalized parahydrogen caused from cyclobutane dimer by DNA crosslinking was investigated.