Development of Photochemical RNA Editing induced by Photothermal Effect

为了提高光交联DNA双链中胞嘧啶的脱氨反应，考察了添加剂对脱氨反应的影响。结果表明，二胺衍生物能以浓度依赖的方式加速反应。在两个胺基之间具有大于四个碳原子距离的二胺衍生物明显地加速了光化学C-U转变反应。对于亚精胺，加速作用是由亚精胺与DNA双链之间的相互作用引起的，而pH的升高对加速作用几乎没有贡献。由于哺乳动物细胞中含有微量的精脒和精胺，因此本研究所证实的光化学C-U转换的加速可能发生在哺乳动物细胞中。这提供了具有更高光编辑活性的人工寡核苷酸的新设计。

To improve the deamination reaction of cytosine in the photocrosslinked DNA double strand, the effect of additives on the deamination reaction was evaluated. The results indicated that diamine derivatives can accelerate the reaction in a concentration dependent manner. diamine derivatives having more than four carbons distance between two amine groups clearly accelerate the photochemical C-U transition reaction. For spermidine, the acceleration effect was caused by the interaction between spermidine and DNA double strand, although the increase of pH scarcely contributed to the acceleration effect. As the mammalian cells contain millimolars of spermidine and spermine, the acceleration of photochemical C-U transition, that was demonstrated in this study, might occur in mammalian cells. This provides a new design of artificial oligonucleotide having higher photo-editing activity.