Development of Photochemical RNA Editing induced by Photothermal Effect

光热效应诱导的光化学RNA编辑研究进展

• 批准号: 23350079
• 负责人: FUJIMOTO KENZO
• 金额: $ 11.98万
• 依托单位: Japan Advanced Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23350079/
• 关键词: 光架橋; RNA編集; RNA操作; 光クロスリンク; 遺伝子変換; 酵素不要; RNA; 光編集

To improve the deamination reaction of cytosine in the photocrosslinked DNA double strand, the effect of additives on the deamination reaction was evaluated. The results indicated that diamine derivatives can accelerate the reaction in a concentration dependent manner. diamine derivatives having more than four carbons distance between two amine groups clearly accelerate the photochemical C-U transition reaction. For spermidine, the acceleration effect was caused by the interaction between spermidine and DNA double strand, although the increase of pH scarcely contributed to the acceleration effect. As the mammalian cells contain millimolars of spermidine and spermine, the acceleration of photochemical C-U transition, that was demonstrated in this study, might occur in mammalian cells. This provides a new design of artificial oligonucleotide having higher photo-editing activity.

为提高光交联型DNA双链中胞嘧啶的脱氨基反应，考察了添加剂对脱氨基反应的影响。结果表明，二胺类化合物对反应有促进作用，且具有浓度依赖性。二胺类化合物中两个胺基团之间的碳间距大于4个的二胺类化合物明显地加速了光化学C-U转变反应。对于亚精胺，加速效应是由亚精胺与DNA双链的相互作用引起的，而pH的升高对加速效应几乎没有贡献。由于哺乳动物细胞中含有毫米级的亚精胺和精胺，本研究表明，哺乳动物细胞中可能发生光化学C-U转换的加速。这为具有更高照片编辑活性的人工寡核苷酸提供了一种新的设计。

项目成果

光架橋形成抑制方法、及び自己架橋体形成抑制型光応答性核酸

抑制光交联形成的方法以及抑制自交联剂形成的光响应性核酸

• 发表时间: 2013

Regulation of gene expression by photocrosslinking antisense oligonucleotide containing of carbazole derivative

含咔唑衍生物的光交联反义寡核苷酸对基因表达的调控

• 发表时间: 2011
• 作者: Atsuo Shigeno;Takashi Sakamoto;Kenzo Fujimoto
• 通讯作者: Kenzo Fujimoto

光で操るDNA, パリティ特集「DNAの物理」

用光操纵DNA，Parity特辑“DNA的物理学”

• 发表时间: 2011
• 作者: T. Okanishi;T. Yoda;Y. Sakiyama;T. Miyahara;K. Miyatake;M. Uchida;M. Watanabe;藤本健造
• 通讯作者: 藤本健造

光化学的なDNA及びRNA操作法の開発

光化学DNA和RNA操作方法的发展

• 发表时间: 2012
• 作者: Takashi Sakamoto;Hikaru Hayakawa;Jyun Sasaki;Satomi Kishi and Kenzo Fujimoto;藤本 健造
• 通讯作者: 藤本 健造

Signal turn-on probe for nucleic acid detection based on 19F nuclear magnetic resonance

• DOI: 10.1016/j.bmcl.2010.11.013
• 发表时间: 2011-01-01
• 期刊: BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
• 影响因子: 2.7
• 作者: Sakamoto, Takashi;Shimizu, Yu-ki;Fujimoto, Kenzo
• 通讯作者: Fujimoto, Kenzo