Investigation of rheological effect on Hepatocyte isolation procedure for liver regeneration

流变学对肝再生肝细胞分离过程影响的研究

• 批准号: 24656129
• 负责人: MIZUNUMA Hiroshi
• 金额: $ 2.25万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24656129/
• 关键词: ﾊﾞｲｵ流体力学; 細胞治療; 移植; せん断; マイクロチャネル; 細胞; 肝臓; せん断流; 細胞移植; 臓器再生

Hepatocyte isolation procedure using a perfusion is one of the most important process for improvements of cell transplantation, developments of an artificial biological liver and establishments of a liver regeneration. In this study, three types of experiments were employed to investigate the rheological effect of the flow on the hepatocyte viabilities and functions during the isolation procedures. The micro-channel experiment imitated the clinical procedures using a needle and catheters was employed to clarify the effect of the shear stress induced near wall region in micro-channel. The rotating disks experiment was employed to investigate the effect of the shear stress intensity and exposure time on the cell viability. Furthermore, the evaluation experiment for the cell function was conducted to find the difference of the metabolisms of ammonia between isolated single cells and clustered cells.

利用灌注法分离肝细胞是改进细胞移植、发展人工生物肝和建立肝再生的重要步骤之一。本研究通过三种不同类型的实验，研究在分离过程中流动对肝细胞存活率和功能的影响。通过模拟临床操作的微通道实验，研究了近壁区剪切应力对微通道内切应力的影响。采用旋转圆盘实验研究了剪切应力强度和暴露时间对细胞存活率的影响。此外，还进行了细胞功能评价实验，找出了分离的单个细胞和聚集的细胞对氨的代谢的差异。

项目成果

Effects of stretching flow on hepatocyte viability for cell transplantation

拉伸流对细胞移植肝细胞活力的影响

• 发表时间: 2013
• 作者: Hiromichi Obara;Sandi Sufiandi;Shin Enosawa Tatsuhide Sumida;Huai-Che Hsu;Naoto Matsuno;Hiroshi Mizunuma
• 通讯作者: Hiroshi Mizunuma

肝実質細胞への流体力学的負荷に関する検討

肝实质细胞的水动力负荷研究

• 发表时间: 2012
• 作者: Sandi Sufiandi;Hiromichi Obara;Tatsuhide Sumida;Huai-Che Hsu,Naoto Matsuno;Shin Enosawa;Hiroshi Mizunuma;隅田竜秀,小原弘道,許懐哲,安田利貴,松野直徒,絵野沢伸,水沼博;小原弘道,隅田竜秀,許懐哲,松野直徒,水沼博,絵野沢伸
• 通讯作者: 小原弘道,隅田竜秀,許懐哲,松野直徒,水沼博,絵野沢伸

細胞移植のための肝実質細胞の力学特性の検討

用于细胞移植的肝实质细胞的机械特性检查

• 发表时间: 2013
• 作者: Sandi Sufiandi;Hiromichi Obara;Tatsuhide Sumida;Huai-Che Hsu,Naoto Matsuno;Shin Enosawa;Hiroshi Mizunuma;隅田竜秀,小原弘道,許壊哲,松野直徒,絵野沢伸,水沼博
• 通讯作者: 隅田竜秀,小原弘道,許壊哲,松野直徒,絵野沢伸,水沼博

細胞移植用肝実質細胞が受けるせん断流による損傷

细胞移植时剪切流对肝实质细胞造成的损伤

• 发表时间: 2012
• 作者: Sandi Sufiandi;Hiromichi Obara;Tatsuhide Sumida;Huai-Che Hsu,Naoto Matsuno;Shin Enosawa;Hiroshi Mizunuma;隅田竜秀,小原弘道,許懐哲,安田利貴,松野直徒,絵野沢伸,水沼博
• 通讯作者: 隅田竜秀,小原弘道,許懐哲,安田利貴,松野直徒,絵野沢伸,水沼博

Improvement of infusion process in cell transplantation : Effect of shear stress on hepatocyte viability under horizontal and vertical syringe orientation

细胞移植输注过程的改进：水平和垂直注射器方向下剪切应力对肝细胞活力的影响

• 发表时间: 2014
• 期刊: Cell medicine
• 作者: Sandi Sufiandi;Hiromichi Obara;Shin Enosawa;Huai-Che Hsu;Naoto Matsuno;Hiroshi Mizunuma
• 通讯作者: Hiroshi Mizunuma