Development of method for determining indoor fungal concentration using genetic analysis from the viewpoint of occupants' health risk assessment

从居住者健康风险评估的角度开发遗传分析测定室内真菌浓度的方法

• 批准号: 24656338
• 负责人: KENICHI Hasegawa
• 金额: $ 2.58万
• 依托单位: Akita Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24656338/
• 关键词: 室内浮遊真菌; 健康リスク; DNA解析; 捕集法; 濃度定量法

In order to collect airborne fungi in indoor, the appication possibility of methodology usign a impinger was tested. Optimum conditions for collection of airborne fungi such as air sampling rate, sampling time and so on were examined. It was revealed that it was not easy to keep the collection efficiency stable during a measurement period. Therefore it is necessary to develop the methdology sampling by filtration in future.In determining indoor fungal concentration using genetic analysis, it was found that the more samples of microbial species were collected, the higher analysis precision was. It is important to design a primer for improvement of amplification efficiency of DNA.

为了在室内收集机载真菌，测试了方法论的应用可能性。检查了收集机载真菌的最佳条件，例如空气采样率，采样时间等。据透露，在测量期间保持收集效率稳定并不容易。因此，有必要通过过滤在未来的过滤中开发甲基化的采样。在使用遗传分析确定室内真菌浓度时，发现收集了更多的微生物物种样本，更高的分析精度为。设计提高DNA扩增效率的底漆很重要。