Time-resolved small-angle X-ray scattering of GUV deformed by F-BAR domain

F-BAR 域变形的 GUV 的时间分辨小角 X 射线散射

• 批准号: 24657078
• 负责人: NOBUTAKA Shimizu
• 金额: $ 2.58万
• 依托单位: High Energy Accelerator Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657078/
• 关键词: 生体膜変形; 時分割X線小角散乱; ストップトフロー; X線溶液散乱; 時分割測定; GUV; 脂質膜変形; X線小角散乱; 生体膜

Some proteins transform an intracellular organelle and maintain structure. It is important to study those interactions in order to understand the structure and the function of the organelle. Then, a stopped-flow was installed in the small-angle X-ray scattering beamline at the Japanese synchrotron facility, Photon Factory, and the structural change of Giant unilamellar vesicle (GUV) interacted by the BAR domain protein was measured by time-resolve method. As a result, although it was very preliminary analysis, it was suggested the structure of GUV was changed with BAR domain in the space scale of around 125 angstrom within 210 seconds.

有些蛋白质可以改变细胞内的细胞器并维持结构。研究这些相互作用对于了解细胞器的结构和功能是很重要的。然后，在日本同步辐射装置光子工厂的小角X射线散射光束线上安装了停流装置，并用时间分辨方法测量了条域蛋白与巨型单层囊泡相互作用的结构变化。因此，虽然这是非常初步的分析，但它表明在210秒内，GUV的结构在大约125埃的空间尺度上发生了杆状结构的改变。

项目成果

Current Status of Small Angle X-ray Scattering Beamlines at Photon Factory

光子工厂小角X射线散射束线现状

• 发表时间: 2012
• 作者: Shimizu;N.;Mori;T.;Igarashi;N.;Ohta;H.;Nagatani;Y.;Kosuge;T. and Ito;K.
• 通讯作者: K.

PF BL-15A：高輝度 XAFS/XRF/SAXS ビームライン

PF BL-15A：高亮度 XAFS/XRF/SAXS 光束线

• 发表时间: 2014
• 作者: 五十嵐教之;小山篤;森丈晴;上條亜衣;西條慎也;大田浩正;仁谷浩明;丹羽尉博;阿部 仁;清水伸隆
• 通讯作者: 清水伸隆

Refurbishment of SAXS beamlines at Photon Factory

Photon 工厂 SAXS 光束线翻新

• 发表时间: 2013
• 作者: 五十嵐教之;小山篤;森丈晴;上條亜衣;西條慎也;大田浩正;仁谷浩明;丹羽尉博;阿部 仁;清水伸隆;Nobutaka Shimizu
• 通讯作者: Nobutaka Shimizu

BL-15A : Semi-microbeam XAFS/XRF and High- brilliance SAXS beamline at Photon Factory

BL-15A：Photon Factory 的半微束 XAFS/XRF 和高亮度 SAXS 光束线

• 发表时间: 2013
• 作者: Igarashi;N.;Koyama;A.;Mori;T.;Ohta;H.;Kamijo;A.;Saijyo;S.;Niwa;Y.;Nitani;H.;Abe;H. and Shimizu;N.
• 通讯作者: N.

Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling.

• DOI: 10.1107/s0909049513020827
• 发表时间: 2013-11
• 期刊: Journal of synchrotron radiation
• 影响因子: 2.5
• 作者: Hirai M;Kimura R;Takeuchi K;Hagiwara Y;Kawai-Hirai R;Ohta N;Igarashi N;Shimuzu N
• 通讯作者: Shimuzu N