Single molecule observation of protein translocation through translocon

在真核细胞中，蛋白质通过内质网上的转位子进行转位是维持细胞生命的重要基础之一。本课题的挑战在于建立一个能够在单分子水平上观察多肽链通过转座子运动的实验系统。我们建立了一个可以同步观察蛋白质跨膜转运的系统，发现只有2-3个带正电荷的氨基酸残基会减慢蛋白质跨膜转运。

Protein translocation through translocon on the endoplasmic reticulum in the eukaryotic cells is one of the most critical bases to maintain the life of the cells. The challenge in this project was to establish the experimental system by which the movement of the polypeptide chain through translocon can be observed at the single molecule level. We have set up the system in which we can observe protein translocation synchronously across the membrane and found that only 2-3 positively charged amino acid residues slow down the movement through translocon.