Integration of membrane proteins through the endoplasmic reticulum translocon and other organelle in the eukaryotic cells

在本项目中，我们研究了真核细胞中蛋白质通过内质网上的转位整合以及膜蛋白靶向其他细胞器。我们演示了以下内容；疏水序列通过转座子的膜整合可通过核糖体调节。多肽链上带正电的氨基酸残基使多肽链的运动停止，然后恢复运动。在天然存在的膜蛋白的膜整合中，可以观察到边缘疏水段的强制膜整合。膜内胆固醇对转位功能有多效性影响。

In this project, we have addressed the protein integration through translocon on the endoplasmic reticulum in the eukaryotic cells and the targeting of membrane proteins to the other organelle. We demonstrated the follows; the membrane integration of hydrophobic sequences though translocon can be regulated by ribosomes. The movement of polypeptide chain is stalled by positively charged amino acid residues on the chain and then resumes. The forced membrane integration of marginally hydrophobic segment can be observed in the membrane integration of naturally occurring membrane protein. Cholesterol in the membrane exerts pleiotropic effects on the translocon function.