Mapping of mutation point on holokinetic chromosome in Bombyx mori

本研究对国家生物资源计划（NBRP）提供的3个家蚕品系（r501、r52和r53）进行了染色体突变分析。BAC-FISH（使用细菌人工染色体的荧光原位杂交）揭示了染色体23和25之间融合的确切方向。我确定相互易位是r52和r53菌株突变的原因。用BAC-FISH技术分析了各突变染色体的双链断裂和进一步融合情况。这些检测到一个桥BAC负责突变染色体。本研究试图通过PCR技术揭示突变位点，为家蚕基因组数据库的建立提供参考。

I have analyzed chromosome mutation in 3 Bombyx mori strains (r501, r52 and r53) provided through national bioresource project (NBRP). BAC-FISH (fluorescence in situ hybridization using bacterial artificial chromosomes) revealed the exact direction of the fusion between chromosomes 23 and 25. I defined reciprocal translocation is the cause for the mutation in r52 and r53 strains. The double strand breakage and further fusion for each mutation chromosomes were also analyzed by the BAC-FISH. These detected a bridge BAC responsible for a mutation chromosome. I am trying to reveal the mutation point by PCR as a reference of Bombyx mori genome databes.