Mapping of mutation point on holokinetic chromosome in Bombyx mori

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I have analyzed chromosome mutation in 3 Bombyx mori strains (r501, r52 and r53) provided through national bioresource project (NBRP). BAC-FISH (fluorescence in situ hybridization using bacterial artificial chromosomes) revealed the exact direction of the fusion between chromosomes 23 and 25. I defined reciprocal translocation is the cause for the mutation in r52 and r53 strains. The double strand breakage and further fusion for each mutation chromosomes were also analyzed by the BAC-FISH. These detected a bridge BAC responsible for a mutation chromosome. I am trying to reveal the mutation point by PCR as a reference of Bombyx mori genome databes.