Integration and visualization of genome information in Lepidoptera

鳞翅目基因组信息的整合和可视化

• 批准号: 15380227
• 负责人: SAHARA Ken
• 金额: $ 8.83万
• 依托单位: HOKKAIDO UNIVERISTY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380227/
• 关键词: Bombyx mori; BAC-FISH; GISH; contig; linkage map; chromosome identification; karyotyping; Lepidoptera; BAC; FISH; RAPD地図; コールドスポット; 性染色体; 突然変異地図

We have developed Bombyx mori sex chromosome identification method and first BAC-FISH system in Lepidoptera by means of W-BAC selected by female specific STSs in 2003. The former method has been applied to resolve multiple sex chromosome systems in 2005. Modified BAC-FISH procedure using genetically mapped B.mori BACs as probes allow us to identify each 28 bivalents. Furthermore, the combination of two to four either green or red fluorescence-labeled probes per chromosome enables us to recognize unequivocally each of the 28 bivalents of the B.mori karyotype by its labeling pattern, which have been impossible for more than 80 years. Through the research we have accomplished 2nd generation molecular linkage map including 512 BAC contigs, which correspond to most unique genes published on the web. These may feasibly facilitate the map-based cloning the genes corresponding to mutants.The BAC-FISH signal localities coincide with their mapping loci detected by genetic analysis with a few exceptions. Since the discrepancy may be caused by mistakes of three-point analysis, we had better re-calculate the results.

2003年，我们利用家蚕雌虫特异性STS筛选出的W-BAC，建立了家蚕性染色体鉴定方法和鳞翅目昆虫的第一个BAC-FISH系统。2005年，前一种方法已被应用于解决多性染色体系统。使用遗传定位的B.mori BAC作为探针的改良BAC-FISH程序允许我们鉴定每28个二价体。此外，每个染色体上2 - 4个绿色或红色荧光标记探针的组合使我们能够通过标记模式明确识别B.mori核型的28个二价体中的每一个，这在80多年来是不可能的。通过研究，我们已经完成了第二代分子连锁图谱，其中包括512个BAC重叠群，这些重叠群与网络上公布的大多数独特基因相对应。BAC-FISH信号位点与遗传分析所测得的定位位点基本一致，但有少数例外。这种差异可能是由于三点分析的错误造成的，我们最好重新计算结果。

项目成果

The genome sequence of silkworm, Bombyx mori

• DOI: 10.1093/dnares/11.1.27
• 发表时间: 2004-02-29
• 期刊: DNA RESEARCH
• 影响因子: 4.1
• 作者: Mita, K;Kasahara, M;Sasaki, T
• 通讯作者: Sasaki, T

Phylogeny of the sex-determining gene Sex-lethal in insects

• DOI: 10.1139/g05-107
• 发表时间: 2006-03-01
• 期刊: GENOME
• 影响因子: 3.1
• 作者: Traut, Walther;Mimi, Teruyuki;Sahara, Ken
• 通讯作者: Sahara, Ken

CDNA cloning, gene structure, and expression of Broad-Complex (BR-C) genes in the silkworm, Bombyx mori

• DOI: 10.1016/j.ibmb.2004.06.005
• 发表时间: 2004-09-01
• 期刊: INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
• 影响因子: 3.8
• 作者: Ijiro, T;Urakawa, H;Fujrwara, Y
• 通讯作者: Fujrwara, Y

Retrotransposable elements on the W chromosome of the silkworm, Bombyx mori

• DOI: 10.1159/000084946
• 发表时间: 2005-01-01
• 期刊: CYTOGENETIC AND GENOME RESEARCH
• 影响因子: 1.7
• 作者: Abe, H;Mita, K;Shimada, T
• 通讯作者: Shimada, T

The Bombyx mori karyotype and the assignment of linkage groups

• DOI: 10.1534/genetics.104.040352
• 发表时间: 2005-06-01
• 期刊: GENETICS
• 影响因子: 3.3
• 作者: Yoshido, A;Bando, H;Sahara, K
• 通讯作者: Sahara, K