Microbial ecology of high-temperature compost and its usefulness as genetic resource

高温堆肥的微生物生态及其作为遗传资源的用途

基本信息

  • 批准号:
    24658075
  • 负责人:
  • 金额:
    $ 2.58万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 财政年份:
    2012
  • 资助国家:
    日本
  • 起止时间:
    2012-04-01 至 2014-03-31
  • 项目状态:
    已结题

项目摘要

Temperature reached over 90 degrees centigrade in the upper position of the compost of the high-temperature composting system. Viable cell numbers by surface plate method was about 1/100 to 1/1000 of those by microscopic observation method, suggesting that unculturable bacteria were important in the high-temperature composting process. Bacillus species dominated in the early stage of composting, accounting for 36% of the total cell numbers, but Melghirimyces thermohalophilus accounted for 63%, indicating that bacterial flora drastically changed in the process of the composting. Genes encoding thermophilic enzymes were directly cloned from DNA isolated from the compost and thermophilic bacteria producing bacterial cell wall-degrading enzymes were isolated from the compost, indicating that the high-temperature compost was quite useful as the genetic resource for isolating thermophilic enzymes and bacteria.
高温堆肥系统堆肥上部温度达到90摄氏度以上。平板法测得的活细胞数约为显微镜观察法的1/100至1/1000,表明不可培养的细菌在高温堆肥过程中很重要。堆肥初期芽孢杆菌属占主导地位,占细胞总数的36%,但嗜热嗜盐梅尔吉氏菌占63%,表明堆肥过程中细菌菌群发生了巨大变化。从堆肥中分离的DNA中直接克隆了编码嗜热酶的基因,并从堆肥中分离出了产生细菌细胞壁降解酶的嗜热细菌,表明高温堆肥作为分离嗜热酶和细菌的遗传资源是非常有用的。

项目成果

期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
高温性堆肥における経時的・空間的温度変化に伴う菌叢変化
嗜热堆肥中与时空温度变化相关的细菌菌群变化
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
    粟冠真紀子;福丸琢人;東俊之;千葉悠佳;木村哲哉;粟冠和郎
  • 通讯作者:
    粟冠和郎
高温性堆肥の経時的・空間的温度変化に伴う菌叢変化
嗜热堆肥中与时空温度变化相关的细菌菌群变化
  • DOI:
  • 发表时间:
    2014
  • 期刊:
  • 影响因子:
    0
  • 作者:
    粟冠真紀子;福丸琢人;東俊之;千葉悠佳;木村哲哉;粟冠和郎
  • 通讯作者:
    粟冠和郎
超高温性堆肥の温度と微生物群の経時的・空間的変化の解析
超高温堆肥温度及微生物群落时空变化分析
  • DOI:
  • 发表时间:
    2013
  • 期刊:
  • 影响因子:
    0
  • 作者:
    福丸琢人;粟冠真紀子;坂井勝;木村哲哉;粟冠和郎
  • 通讯作者:
    粟冠和郎
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SAKKA Kazuo其他文献

SAKKA Kazuo的其他文献

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{{ truncateString('SAKKA Kazuo', 18)}}的其他基金

Study on the mechanism by which yellow substance increases enzymatic cellulose degradation and isolation of novel yellow substance
黄色物质促进纤维素酶降解的机理研究及新型黄色物质的分离
  • 批准号:
    25292049
  • 财政年份:
    2013
  • 资助金额:
    $ 2.58万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Studies on structure and function of bacterial yellow affinity substance capable of enhancing enzymatic degradation of cellulose and its gene
增强纤维素酶降解细菌黄色亲和物质及其基因的结构与功能研究
  • 批准号:
    21380197
  • 财政年份:
    2009
  • 资助金额:
    $ 2.58万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Structure of Secondary Cell Wall Polymers in Cell Surfaces of Anaerobes and Interaction between Them and SLH Domains
厌氧菌细胞表面次生细胞壁聚合物的结构及其与SLH结构域的相互作用
  • 批准号:
    15380060
  • 财政年份:
    2003
  • 资助金额:
    $ 2.58万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

相似海外基金

17-ERACoBioTech Thermophilic bacteria and archaeal chassis for extremolyte production -HotSolute
17-ERACoBioTech 用于生产极电解质的嗜热细菌和古菌底盘 -HotSolute
  • 批准号:
    BB/R02166X/1
  • 财政年份:
    2018
  • 资助金额:
    $ 2.58万
  • 项目类别:
    Research Grant
Analysis of mesophilic cyanobacterium mutant strain that has acquired a high temperature tolerance comparable to moderately thermophilic bacteria
获得与中度嗜热细菌相当的耐高温性的嗜温蓝藻突变株的分析
  • 批准号:
    15K07368
  • 财政年份:
    2015
  • 资助金额:
    $ 2.58万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular and metabolic characterization of uncultivated thermophilic bacteria from Canadian hot springs with applications to biotechnology
加拿大温泉中未培养的嗜热细菌的分子和代谢特征及其在生物技术中的应用
  • 批准号:
    405223-2011
  • 财政年份:
    2012
  • 资助金额:
    $ 2.58万
  • 项目类别:
    Postdoctoral Fellowships
Quantitative Analysis of Metabolic Networks and Flux Dynamics in Thermophilic Bacteria
嗜热细菌代谢网络和通量动力学的定量分析
  • 批准号:
    1120684
  • 财政年份:
    2011
  • 资助金额:
    $ 2.58万
  • 项目类别:
    Continuing Grant
Molecular and metabolic characterization of uncultivated thermophilic bacteria from Canadian hot springs with applications to biotechnology
加拿大温泉中未培养的嗜热细菌的分子和代谢特征及其在生物技术中的应用
  • 批准号:
    405223-2011
  • 财政年份:
    2011
  • 资助金额:
    $ 2.58万
  • 项目类别:
    Postdoctoral Fellowships
Engineering of proteins from thermophilic bacteria as a scaffold for thermally tolerant artificial enzyme
嗜热细菌蛋白质工程作为耐热人工酶的支架
  • 批准号:
    18560753
  • 财政年份:
    2006
  • 资助金额:
    $ 2.58万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Biodesulfurization using thermophilic bacteria and identification of bds genes
嗜热菌生物脱硫及bds基因鉴定
  • 批准号:
    13650859
  • 财政年份:
    2001
  • 资助金额:
    $ 2.58万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
好熱性細菌におけるアミノ酸生合成系に関わる酵素群の活性発現・制御の分子機構
嗜热菌氨基酸生物合成酶表达与调控的分子机制
  • 批准号:
    99J09447
  • 财政年份:
    1999
  • 资助金额:
    $ 2.58万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
NSF/EPA TSE: Novel Compressed Solvent Extraction Processes for Enhanced Biomass Conversion by Thermophilic Bacteria
NSF/EPA TSE:用于增强嗜热细菌生物质转化的新型压缩溶剂萃取工艺
  • 批准号:
    9817069
  • 财政年份:
    1999
  • 资助金额:
    $ 2.58万
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    Continuing Grant
Structure and function of two novel respiratory chain complex from Gram-positive thermophilic bacteria
革兰氏阳性嗜热菌两种新型呼吸链复合物的结构和功能
  • 批准号:
    10680617
  • 财政年份:
    1998
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    $ 2.58万
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    Grant-in-Aid for Scientific Research (C)
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