Identification of target proteins for ubiquitination in brown adipose tissue of fasted rat

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To clarify the mechanism for energy metabolism regulation by specific protein degradation, we attempt to identify the proteins that ubiquitinated in response to energy level in rat brown adipose tissue. Male Wistar rats aged 7 weeks old were subjected to 1 week restricted feeding, and then divided into 2 groups, fed and fasted. The rats were administrated epoxomicin, a potent proteasome inhibitor. The dose and method for administration of epoxomicin were determined to detect the difference of ubiquitinated protein levels between the 2 groups.