Analysis of the mechanisms of phosphorus homeostasis in major organ using high-phosphorus diet administrated rat model and its application

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Ten male Wistar rats were divided into two groups and fed a diet containing 0.3% (C) or 1.2% (HP) phosphorous for 24 days. DNA microarray analyses were performed using kidney, liver and white adipose tissue (WAT) of these animals. A hierarchical clustering analysis revealed that gene expression profiles of the two groups are different from each other. Among the genes that were differentially expressed between the two groups, there was significant upregulation of type IIb sodium-dependent phosphate transporter (NaPi-IIb) in the kidney of HP rats. As for the liver, upregulation of fatty acid beta-oxidation and downregulation of amino acid catabolic process by HP diet administration were observed. Upregulation of genes related to fatty acid beta-oxidation was also observed in the WAT of HP rat. Thus, our results indicate that administration of high phosphorus diet induced not only regulation of phosphate homeostasis but also changes in various metabolic pathways.