Analysis of the mechanisms of phosphorus homeostasis in major organ using high-phosphorus diet administrated rat model and its application

高磷饮食大鼠模型分析主要器官磷稳态机制及其应用

• 批准号: 23300273
• 负责人: NAKAI Yuji
• 金额: $ 11.73万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23300273/
• 关键词: 高リン食; DNAマイクロアレイ; ラット; 腎臓; 肝臓; 白色脂肪組織; NaPi-IIb; 脂質代謝; 炎症; FGF21; 脂肪酸組成; エネルギー代謝; 遺伝子発現

Ten male Wistar rats were divided into two groups and fed a diet containing 0.3% (C) or 1.2% (HP) phosphorous for 24 days. DNA microarray analyses were performed using kidney, liver and white adipose tissue (WAT) of these animals. A hierarchical clustering analysis revealed that gene expression profiles of the two groups are different from each other. Among the genes that were differentially expressed between the two groups, there was significant upregulation of type IIb sodium-dependent phosphate transporter (NaPi-IIb) in the kidney of HP rats. As for the liver, upregulation of fatty acid beta-oxidation and downregulation of amino acid catabolic process by HP diet administration were observed. Upregulation of genes related to fatty acid beta-oxidation was also observed in the WAT of HP rat. Thus, our results indicate that administration of high phosphorus diet induced not only regulation of phosphate homeostasis but also changes in various metabolic pathways.

雄性Wistar大鼠10只，随机分为两组，分别饲喂含磷0.3%(C)和1.2%(HP)的饲料24天。使用这些动物的肾脏、肝脏和白色脂肪组织(WAT)进行DNA微阵列分析。等级聚类分析表明，两组的基因表达谱彼此不同。在两组差异表达的基因中，Hp大鼠肾脏IIb型钠依赖磷酸转运蛋白(Napi-IIb)显著上调。在肝脏方面，观察到HP饮食可上调脂肪酸β-氧化，下调氨基酸分解代谢过程。在HP大鼠的WAT中也观察到与脂肪酸β-氧化相关的基因上调。因此，我们的结果表明，高磷饮食不仅诱导了磷酸盐稳态的调节，而且还改变了各种代谢途径。

项目成果

Current status and case studies in nutrigenomics

营养基因组学的现状和案例研究

• 发表时间: 2012
• 作者: Hariya;N.ら;高村 仁知;中井雄治
• 通讯作者: 中井雄治

The Novel Mechanism of Phosphorus Homeostasis -A DNA Microarray Analysis in High-Phosphorus Diet Administrated Rat Kidney

磷稳态的新机制——高磷饮食大鼠肾脏的DNA微阵列分析

• 发表时间: 2011
• 作者: Omoto S;Tazumi S;Yoshida R;Nagai H;Yoshida K;Kudo R;Hatake K;Morimoto K;中井雄治
• 通讯作者: 中井雄治

ニュートリゲノミクス研究の最適化

优化营养基因组学研究

• 发表时间: 2012
• 作者: 中井雄治

高リン食摂取ラット腎臓の遺伝子発現に対するホエイタンパク質の影響

乳清蛋白对高磷酸盐饮食大鼠肾脏基因表达的影响

• 发表时间: 2012
• 作者: 林ちひろ;石島 智子;阿部 啓子;中井雄治
• 通讯作者: 中井雄治

Influence of a short-term iron-deficient diet on hepatic gene expression profiles in rats.

• DOI: 10.1371/journal.pone.0065732
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kamei A;Watanabe Y;Kondo K;Okada S;Shinozaki F;Ishijima T;Nakai Y;Kondo T;Arai S;Abe K
• 通讯作者: Abe K