Investigation of target proteins by isothiocyanate with fluorescent probe

虽然异硫氰酸酯具有抗癌、抗菌等生物学功能，但其作用机制尚不清楚。我们在生理条件下发现了ITC与赖氨酸的共价内合。在本研究中，我们主要利用FITC来研究ITC的荧光特性。制备并鉴定了一种荧光结构单克隆抗体，并将其应用于细胞荧光染色。然后用荧光扫描或特异性抗体在凝胶中检测多个ITC内合蛋白。ITC内合蛋白的鉴定仍有待完成。

Though isothiocyanates have some biological functions such as anti-cancer or anti-bacterial activities, the mechanisms has not been shown in detail. We found the covalent adduction of ITC with lysine in a protein at physiological conditions. In this study, we focused in fluorescent character of ITC by using FITC. A monoclonal antibody to fluorescent structure has been prepared and characterized and then applied to fluorescent staining of cells. The multiple ITC adducted proteins were then detected in a gel by fluorescent scanning or by specific antibody. The identification of ITC adducted proteins remains to be done.