Fine structure of polysaccharides obtained from dietary fibers.

从膳食纤维中获得的多糖的精细结构。

基本信息

批准号：
03680054
负责人：
KATO Yoji
金额：
$ 1.34万
依托单位：
Hirosaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03680054/
关键词：
Dietary fiber Xyloglucan Inulin Edible burdock Polysacchride Diffusion speed Leaf vegetables Root vegetables フラクタンペクチン

项目摘要

From the viewpoint of the physiological function of dietary fiber, it is important to elucidate the structure and properties of cell wall polysaccharides. In this research project, we obtained the following results.(1) Carbohydrates in leaf vegetables (cabbage, chainease cabagge, spinach, chingentsuai and lettuce) and root vegetables (turnip, japanese radish, edible burdock, carrot and east indian lotus) were compared in triturated, freeze-dried samples. Fractionation of the carbohydrates, and the sugar composition an sugar-linkage composition analyzes of the fractions suggested that their basic constituent polysaccharides were almost the same. The cell walls consisted of rhamnogalacturonan with side chains, (1->5)-linked arabinan, (1->4)-linked galactan, arabinogalactan, xyloglucan and cellulose.(2) A xyloglucan was isolated from 24% KHO extract of gobo (edible burdock). A methylation analysis and enzymic degradation studies on the polysaccharide showed that gobo-xyloglucan was built … More up predominantly of repeating-oligosaccharide units of hepta-(Glc : Xyl=4 : 3), nona-(Glc : Xyl : Gal : Fuc=4 : 3 : 1 : 1) and deca-(Glc : Xyl : Gal : Fuc=4 : 3 : 2 : 1)saccharides in an approximate molar ratio of 14 : 12 : 5, which are the typical structural units of dicot xyloglucans.(3) The chemical structures of xyloglucans obtained from cabbage, chainease cabagge, spinach, chingentsuai and lettuce were compared. Their basic structures were almost the same.(4) Inulin in edible burdock was hydrolyzed in vivo by enzyme(s) to yield lower molecular weight carbohydrates during storage or heating.(5) The glucose diffusion speed decreased in vitro as the concentration of the water insoluble dietary fiber increased. This was considered to be due to the permiability of the water-insoluble dietary fiber to lower-molecular weight carbohydrates.(6) A glucuronoxylan was isolated from jojoba hull. Methylation analysis and enzymic degradation studies of the polysaccharide showed that jojoba hull xylan has a linear chain of (1->4)-D-xylosyl residues in the backbone, about 13% of which are branched at 0-2 position, with 4-0-methyl-alpha-D-glucopyranosyluronic acid residues.(P.T.O.) Less

从饮食纤维的物理功能的角度来看，阐明细胞壁多糖的结构和特性很重要。在该研究项目中，我们获得了以下结果。（1）在叶蔬菜（卷心菜，链球卡木薯，菠菜，辣椒，chinentsuai和生菜）中的碳水化合物和根蔬菜（萝卜，日本萝卜，可食用的伯恩多克，胡萝卜，胡萝卜和东印度莲花）在三尿中进行了比较。碳水合物的分馏和糖成分的糖链成分分析表明它们的基本组成多糖几乎相同。细胞壁由带有侧链（1-> 5）连接的Arabinan，（1-> 4）连接的Galactan，Arabinogalactan，Xyloglucan和纤维素的鼠李糖酸酯组成。 A methylation analysis and enzymic degradation studies on the polysaccharide shown that gobo-xyloglucan was built … More up predominantly of repeating-oligosaccharide units of hepta-(Glc: Xyl=4: 3), nona-(Glc: Xyl: Gal: Fuc=4: 3: 1: 1) and deca-(Glc: Xyl: Gal: Fuc=4: 3: 2: 1）糖含量约为14：12：5，是二甲木葡萄糖的典型结构单位。它们的基本结构几乎是相同的。（4）可食用伯多氏菌中的原蛋白通过酶在体内水解（S）在储存或加热过程中产生较低的分子量碳氢化物。（5）随着水的浓度，葡萄糖扩散速度在体外降低。这被认为是由于水不溶的饮食纤维对低分子量的碳氢化物的许可。（6）从霍霍巴船体中分离出葡萄糖醛酸。多糖的甲基化分析和酶促降解研究表明，霍霍巴船体木制的线性链具有（1-> 4）-d-木糖基在骨架中保留的线性链，其中约13％在0-2位置分支，在0-2位置分支，具有4-0-甲基 - 甲基-Alpha-dlpha-dlpha-alpha-dlpha-dlpha-dlpha-dlpha-dlpha-dlpha-dlpha-dlpha-dlpha-dlpha-lucopopopopopopopopopopopopyranosycycipues。