A novel mechanism model for producing maturation-inducing steroid in fish

一种在鱼类中产生诱导成熟类固醇的新机制模型

• 批准号: 24658165
• 负责人: ADACHI Shinji
• 金额: $ 2.5万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658165/
• 关键词: 水産学; 生理学; 最終成熟; サクラマス; 卵成熟

CR20bHSD has been believed to be the enzyme responsible for DHP (maturation-inducing steroid) production during oocyte maturation in fish. The present study demonstrated that CR20bHSD is not responsible for DHP production and identified a novel enzyme, termed hsd17b3like, as the genuine enzyme responsible for conversion of 17aOHP to DHP during final oocyte maturation in masu salmon. The expression pattern of the mRNA was well matched with serum DHP level. Incubated granulosa cells, just before oocyte maturation period, could convert efficiently exogenous 17aOHP to DHP as well as increasing the mRNA levels when incubated with gonadotropic preparations. These results clearly indicated that hsd17b3like is the enzyme responsible for DHP production during oocyte maturation in the masu salmon, and not CR20bHSD. The present study clearly demonstrated the molecular mechanisms underlying DHP production during oocyte maturation in the masu salmon.

CR20bHSD被认为是鱼类卵母细胞成熟过程中产生DHP(成熟诱导类固醇)的酶。本研究证明CR20bHSD不负责DHP的产生，并鉴定了一种新的酶，命名为hsd17b3，它是真正的酶，负责在Masu鲑鱼卵母细胞的最终成熟过程中将17aOHP转化为DHP。该基因的表达模式与血清DHP水平相吻合。在卵母细胞成熟期前孵育的颗粒细胞能有效地将外源17aOHP转化为DHP，并能增加促性腺激素制剂的mRNA水平。这些结果清楚地表明，在玛苏鲑鱼卵母细胞成熟过程中，hsd17b3like是负责DHP产生的酶，而不是CR20bHSD。本研究清楚地揭示了玛苏鲑鱼卵母细胞成熟过程中产生DHP的分子机制。

项目成果

サクラマスの卵成熟誘起ステロイド産生を担う20βHSD遺伝子の同定

鉴定负责樱桃鲑鱼卵母细胞成熟诱导类固醇产生的 20βHSD 基因

• 发表时间: 2013
• 作者: 倉田 修;和田新平;松山知正;坂井貴光;高野倫一;中易千早;竹澤野葉・井尻成保(北大院水)・柴田安司(基生研)・風藤行紀・鈴木博史(水研セ増養殖研)・足立伸次(北大院水)・山内晧平・長濱嘉孝(愛媛大南水研)
• 通讯作者: 竹澤野葉・井尻成保(北大院水)・柴田安司(基生研)・風藤行紀・鈴木博史(水研セ増養殖研)・足立伸次(北大院水)・山内晧平・長濱嘉孝(愛媛大南水研)

サクラマスの卵成熟誘起ステロイド産生を担う20betaHSD遺伝子の同定

鉴定负责樱桃鲑鱼卵母细胞成熟诱导类固醇产生的 20betaHSD 基因

• 作者: Shigeho Ijiri;Yasushi Shibata;Nonoha Takezawa;Yukinori Kazeto;Naoki Takatsuka;Erika Kato;Yuichi Ozaki;Shinji Adachi;Kohei Yamauchi and Yoshitaka Nagahama.;竹澤野葉
• 通讯作者: 竹澤野葉

Identification of a novel type of 20beta-hydroxysteroid dehydrogenase responsible for maturation-inducing hormone production during oocyte maturation in masu salmon

鉴定一种新型 20β-羟基类固醇脱氢酶，负责马苏鲑鱼卵母细胞成熟过程中成熟诱导激素的产生

• 发表时间: 2013
• 作者: Shigeho Ijiri;Yasushi Shibata;Nonoha Takezawa;Yukinori Kazeto;Naoki Takatsuka;Erika Kato;Yuichi Ozaki;Shinji Adachi;Kohei Yamauchi and Yoshitaka Nagahama
• 通讯作者: Kohei Yamauchi and Yoshitaka Nagahama