Biosynthtic investigation of the didemnins

二德明宁的生物合成研究

• 批准号: 24658181
• 负责人: SAKAI Ryuichi
• 金额: $ 2.58万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658181/
• 关键词: didemnin; Tistrella mobilis; biosynthesis; symbiosys; ジデムニン; 生合成; ホヤ; 共生微生物; 海洋天然物; ペプチド; 非リボゾーム型ペプチド合成酵素; ポリケチド合成酵素

The antitumor peptide didemnins originally found from the Caribbean tunicate Trididemnum solidum were recently identified in the cultured marine bacterium Tistrella mobilis collected in various regions in the world. We thus compared the production and genetic profiles between the tunicate and the microbe. We found that the metabolic pattern in the bacteria differed largely from that of the tunicate. Analysis of microbial fauna in the tunicate indicated that a genus of cyanobacteria is a main associate of the tunicate, but T. mobilis was found as only a minor microbe that associate with the host.

最近，在世界各地收集的培养的海洋细菌中发现了抗肿瘤肽Dodemnins最初从加勒比皮式Trididemnum固体发现的抗肿瘤肽。因此，我们比较了皮象和微生物之间的生产和遗传特征。我们发现，细菌中的代谢模式与受皮象的新陈代谢差异很大。在皮纸酸酯中对微生物动物区系的分析表明，蓝细菌属是剪裁曲酸酯的主要助理，但发现Mobilis是与宿主相关的次要微生物。