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Sponge-derived neurotoxic substances : Structure, Bioactivity, and their cellular localization.

海绵衍生的神经毒性物质：结构、生物活性及其细胞定位。

基本信息

批准号：
17380125
负责人：
SAKAI Ryuichi
金额：
$ 7.17万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

In the present study, we identified new polyamines from a Okinawan sponge Axinissa aculeate that modulate ligand binding to NMDA type glutamate receptors. These polyamines may also function as a factor involved in silica biomineralization in the sponge. From the same extract, we identified new peptides containing polyamine in the substructure and named aculeines. This novel highly modified peptide showed strong convulsant actions in mice. We also isolated Cribropurine, a new purine from a Micronesian sponge Chribrocharina olemda. Cribroprine elicited a seizure-like spontaneous current in rat hippocampal neuron. A lipophilic extract of the sponge Raspailia sp. contained raspailol as seizure-inducing factor in mice. Okinawan sponge Spongosolites afforded an isolation of protein toxin Soritesidine. A terminal amino acid sequence for sotitesidine was determined We identified a cell where an excitatory amino acid dysiherbaine was localized. The cell was identified to be a cyanobacteria Synechosystis sp based on its 16S rDNA sequence This result suggested that dysiherbaine was a cyanobacterial product This species also contained 6-phospholylated deoxynojirimycin derivatives. Investigation of an aqueous extract that exhibited AMPAKine like activity resulted in separation of bioactive fractions. One of the active fractions was shown to contain lectin based on its hemagglutinating activity. The lectin- containing fraction showed AMPAKine-like activity whereas the fraction containing small molecule was responsible for seizure-induction in mice. The preset study demonstrated that toxic extracts that induce convulsion in mice is a interesting source of neuron active compounds that can be useful tools in neurobiology.

在本研究中，我们从冲绳海绵Axinissa aculeate中鉴定了新的多胺，它可以调节配体与NMDA型谷氨酸受体的结合。这些多胺也可能是参与海绵中二氧化硅生物矿化的一个因素。从相同的提取物中，我们鉴定了亚结构中含有多胺的新多肽，并将其命名为针叶。这种新型的高度修饰的多肽在小鼠中表现出很强的惊厥作用。我们还从密克罗尼西亚海绵Chribrocharina olemda中分离出一种新的嘌呤--克里布罗嘌呤。克里溴普林在大鼠海马神经元上诱发癫痫样自发电流。海绵Raspailia sp.的一种亲脂提取物。含有雷帕洛尔，可作为小鼠癫痫的诱发因子。冲绳海绵提供了一种分离蛋白毒素山梨糖苷的方法。测定了Sotitesidine的末端氨基酸序列。我们鉴定了一个兴奋性氨基酸Dysiherbaine所在的细胞。根据其16S rDNA序列，该细胞被鉴定为一株蓝藻，这一结果表明Dysiherbaine是蓝藻的产物，该物种还含有6-磷酸化脱氧诺吉霉素衍生物。对一种表现出AMPAKine样活性的水提取物进行了研究，结果分离出了生物活性部分。根据其血凝活性，其中一个活性部位被证明含有凝集素。含有凝集素的部分显示出AMPAKine样活性，而含有小分子的部分负责诱导小鼠癫痫发作。这项预先设定的研究表明，引起小鼠惊厥的有毒提取物是神经活性化合物的有趣来源，可以作为神经生物学的有用工具。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Marine Molecular Biotechnology Marine Toxins as Research Tools

海洋分子生物技术海洋毒素作为研究工具

DOI：
发表时间：
期刊：
影响因子：
0
作者：
Satoko;Matsunaga;Ryuichi;Sakai;Mitsuru;Jimbo;Hisao;Kamiya;Swanson G.T. and Sakai R.;Swanson G.T.and Sakai R.
通讯作者：
Swanson G.T.and Sakai R.

Cellular origin of dysiherbaine,an excitatory amino acid derived from amarine sponge

地西尔巴因的细胞起源，一种从青色海绵中提取的兴奋性氨基酸