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Sponge-derived neurotoxic substances : Structure, Bioactivity, and their cellular localization.

海绵衍生的神经毒性物质：结构、生物活性及其细胞定位。

基本信息

批准号：
17380125
负责人：
SAKAI Ryuichi
金额：
$ 7.17万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

In the present study, we identified new polyamines from a Okinawan sponge Axinissa aculeate that modulate ligand binding to NMDA type glutamate receptors. These polyamines may also function as a factor involved in silica biomineralization in the sponge. From the same extract, we identified new peptides containing polyamine in the substructure and named aculeines. This novel highly modified peptide showed strong convulsant actions in mice. We also isolated Cribropurine, a new purine from a Micronesian sponge Chribrocharina olemda. Cribroprine elicited a seizure-like spontaneous current in rat hippocampal neuron. A lipophilic extract of the sponge Raspailia sp. contained raspailol as seizure-inducing factor in mice. Okinawan sponge Spongosolites afforded an isolation of protein toxin Soritesidine. A terminal amino acid sequence for sotitesidine was determined We identified a cell where an excitatory amino acid dysiherbaine was localized. The cell was identified to be a cyanobacteria Synechosystis sp based on its 16S rDNA sequence This result suggested that dysiherbaine was a cyanobacterial product This species also contained 6-phospholylated deoxynojirimycin derivatives. Investigation of an aqueous extract that exhibited AMPAKine like activity resulted in separation of bioactive fractions. One of the active fractions was shown to contain lectin based on its hemagglutinating activity. The lectin- containing fraction showed AMPAKine-like activity whereas the fraction containing small molecule was responsible for seizure-induction in mice. The preset study demonstrated that toxic extracts that induce convulsion in mice is a interesting source of neuron active compounds that can be useful tools in neurobiology.

在本研究中，我们确定了新的多胺从冲绳海绵Axinissa aculeate调节配体结合到NMDA型谷氨酸受体。这些多胺也可能作为参与海绵中二氧化硅生物矿化的因素起作用。从相同的提取物中，我们确定了新的肽含有多胺的子结构和命名aculeines。这种新的高度修饰的肽在小鼠中显示出强烈的惊厥作用。我们还分离到一种新的嘌呤，Cribropurine，从一种海绵Chribrocharina olemda。在大鼠海马神经元上，溴普林可诱发一种类似于神经元自发电流的电流。海绵Raspailia sp.的亲脂性提取物含有raspailol作为小鼠中的致炎因子。从冲绳海绵Spongosolites中分离到蛋白毒素Soritesidine。sotitesidine的末端氨基酸序列被确定。我们确定了一个细胞，其中兴奋性氨基酸dysiherbaine被定位。根据16 SrDNA序列分析，该细胞为一种蓝藻Synechosystissp.该结果表明，dysiherbaine是蓝藻的产物，该种细胞中还含有6-磷酸化脱氧野尻霉素衍生物。对表现出AMPAKine样活性的水性提取物的研究导致生物活性级分的分离。根据其血凝活性，其中一种活性组分被证明含有凝集素。含有凝集素的级分显示出AMPAK样活性，而含有小分子的级分负责小鼠中的促炎诱导。这项研究表明，诱导小鼠惊厥的有毒提取物是神经元活性化合物的一个有趣来源，可以成为神经生物学的有用工具。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Marine Molecular Biotechnology Marine Toxins as Research Tools

海洋分子生物技术海洋毒素作为研究工具

DOI：
发表时间：
期刊：
影响因子：
0
作者：
Satoko;Matsunaga;Ryuichi;Sakai;Mitsuru;Jimbo;Hisao;Kamiya;Swanson G.T. and Sakai R.;Swanson G.T.and Sakai R.
通讯作者：
Swanson G.T.and Sakai R.

Cellular origin of dysiherbaine,an excitatory amino acid derived from amarine sponge

地西尔巴因的细胞起源，一种从青色海绵中提取的兴奋性氨基酸