Enzymatic asymmetric cleavage of aquatic products-derived carotenoids and the bio-modulating mechanism of the enzymolysis products

水产品类胡萝卜素的酶促不对称裂解及其酶解产物的生物调节机制

• 批准号: 24658184
• 负责人: HIRATA Takashi
• 金额: $ 2.58万
• 依托单位: Shijonawate Gakuen University (2013)Kyoto University (2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658184/
• 关键词: 酵素分解; 機能性; カロテノイド; 機能性成分

CMO2, betaCarotene 9, 10 monooxygenase, exhibits wide substrate specificity, and it can metabolize xanthophyll as well as betacarotene. We investigated whether CMO2 could cleave aquatic products derived carotenoids through evaluating CMO2 activity in mouse liver homogenate, the activity of recombinant CMO2, and CMO2 activity using CMO2 expressing cultured cells. We, however, could not detect the CMO2 activity in mouse liver homogenate. Although recombinant human CMO2 was produced by Baculotechnologies Company Limited, enzymatically active CMO2 was not obtained. According to a previous report, MDA MB 231, human breast cancer cell line, expresses CMO2, and then, we used this cell line in order to evaluate the CMO2 activity. We could detect no CMO2 metabolites accumulated in carotenoid treated MDA MB 231.

CMO 2是β-胡萝卜素9，10单加氧酶，具有广泛的底物特异性，它可以代谢叶黄素和β-胡萝卜素。我们通过评价小鼠肝匀浆中的CMO 2活性、重组CMO 2的活性以及使用表达CMO 2的培养细胞的CMO 2活性来研究CMO 2是否可以切割水产品来源的类胡萝卜素。然而，我们不能检测到小鼠肝匀浆中的CMO 2活性。虽然重组人CMO 2由Baculotechnologies Company Limited生产，但未获得酶活性CMO 2。根据先前的报道，MDA MB 231，人乳腺癌细胞系，表达CMO 2，然后，我们使用该细胞系以评价CMO 2活性。在类胡萝卜素处理的MDA MB 231中，我们没有检测到CMO 2代谢产物的积累。