Studies on evaluation of glutathione distribution in fish and shellfish and elucidation of its postharvest behavior

鱼类和贝类中谷胱甘肽分布的评价及其采后行为的阐明

• 批准号: 08456104
• 负责人: HIRATA Takashi
• 金额: $ 4.74万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08456104/
• 关键词: Glutathione; glutathione disulfide; functional component; postharvest storage; GSH; 総グルタチオン; 魚介類; ハマチ; ホタテガイ; カキ

Glutathione (GSH) and glutathione disulfide (GSSG) in ca.50 species of fish and shellfish were determined. Except for several species, the contents of GSSG were less than 10% of GSH.Dark meats had 2-3 times higher concentration of total glutathiones (GSH+GSSG, total GSH) than ordinary meats. In general, white meat fish had lower contents of both forms of GSH than red meat fish. Especially, angler, a fish slow in movement, had extremely low contents of GSH and GSSG.Total GSH levels in pelagic fish were relatively high, compared with benthic fish. Of pelagic fich, yellowtail and salmon had more than 200nmol/g of total GSH.Thus fish and shellfish living in the environmental and physiological conditions which active oxygen readily generate, have generally high contents of total GSH, except for shellfish, which have high contents of total GSH (>100nmol/g). Both GSH and GSSG in fish and shellfish showed higher stability in anaerobic conditions than in air. Total GSH also decrease in parallel with GSH in the air condition, suggesting that GSH stored in air does not always convert into GSSG during storage. Scallop showed very high level of total GSH, but an only limited activity of glutathione peroxidase. Further studies are required to clarify the degradation mechanism of GSH during the postharvest storage

测定了50余种鱼类和贝类中的谷胱甘肽（GSH）和谷胱甘肽二硫醚（GSSG）含量。除个别品种外，GSSG含量均低于GSH的10%。黑肉中总谷胱甘肽（GSH+GSSG，总谷胱甘肽）的含量是普通肉类的2 ~ 3倍。总的来说，白肉鱼的两种谷胱甘肽含量都低于红肉鱼。尤其是行动缓慢的垂钓鱼，其GSH和GSSG含量极低。与底栖鱼类相比，远洋鱼类的总谷胱甘肽水平相对较高。在远洋鱼类中，黄尾鱼和鲑鱼的总谷胱甘肽含量超过200nmol/g。因此，生活在活性氧容易产生的环境和生理条件下的鱼类和贝类，其总谷胱甘肽含量普遍较高，但贝类总谷胱甘肽含量较高（100 ~ 100nmol/g）。鱼类和贝类的GSH和GSSG在厌氧条件下比在空气中表现出更高的稳定性。总谷胱甘肽也与空气条件下的谷胱甘肽平行下降，这表明储存在空气中的谷胱甘肽在储存过程中并不总是转化为谷胱甘肽。扇贝的总谷胱甘肽含量很高，但谷胱甘肽过氧化物酶活性有限。采后贮藏过程中谷胱甘肽的降解机制有待进一步研究