Development of immune-response free nano-particles using fungal biosurfactant protein

虽然纳米颗粒已被开发用于医学治疗，但免疫反应，包括巨噬细胞的吞噬作用，是医学应用的主要障碍。近年来，一种来自病原菌烟曲霉分生孢子的生物表面活性剂蛋白疏水蛋白被证明是一种逃避宿主免疫系统的因子（隐身因子）。在本研究中，我们开发了一种新型的以工业真菌米曲霉疏水蛋白RolA包被的隐身纳米颗粒。用纯化的RolA包覆Fe3O4纳米颗粒。当rola包被的颗粒与树突状细胞孵育时，树突状细胞的细胞因子产生不受刺激。rola包被颗粒的巨噬细胞吞噬水平低于未包被颗粒。当rola包被颗粒通过静脉注射给小鼠时，通过mri成像显示，rola包被颗粒在肝脏和脾脏的积聚水平明显低于未包被颗粒。

Although nano-particles have been developed for medical treatments, immune-responses including phagocytosis by macrophages are a major obstacle for medical use. Recently, a biosurfactant protein, hydrophobin originating from conidia of the pathogenic fungus Aspergillus fumigatus has been shown as an escaping factor from host immune-systems (stealth factor). In the present study, we developed novel stealth nano particles coated with the hydrophobin RolA of the industrial fungus Aspergillus oryzae. Fe3O4 nano-particles were coated with purified RolA. When RolA-coated particles were incubated with dendritic cells, cytokine production from dendritic cells was not stimulated. RolA-coated particles showed lower levels of phagocytosis by macrophages than non-coated particles. When RolA-coated particles were administered to mice by intravenous injection, RolA-coated particles indicated significantly lower levels of accumulation to the liver and spleen than non-coated particles by MRI-imaging.