Comprehensive study of fungal biosurfactant proteins that recruit polymer-degrading enzymes onto solid polymer-surfaces

在以纤维素和角蛋白为固体聚合物底物的工业真菌米曲霉上，分离到两种附着在固体表面的小分子生物表面活性蛋白(分子量20 kDa)。克隆了两个编码这两种蛋白的基因，并在米曲霉中高效表达。这两种纯化的生物表面活性蛋白附着在纤维素表面，募集了木聚糖酶F3(XynF3)，该酶缺乏一个碳水化合物结合结构域。在体外，分子相互作用分析表明，固定在固体表面的两种蛋白质具有解离常数Kd。10μM订单到XynF3.我们还分析了酯酶与附着在两种固体材料上的另一种表面活性蛋白质rola的相互作用。

hen the industrial fungus Aspergillus oryzae was grown on cellulose and keratin as solid polymer substrates, two small bio-surfactant proteins (Mw <20 kDa)that were attached to the solid surfaces were isolated. Two genes encoding the two proteins were cloned and highly expressed in A. oryzae. The two purified bio-surfactant proteins attached to the surface of cellulose recruited xylanase F3 (XynF3)which lacks a carbohydrate biding domain. In vitro, molecular interaction analysis revealed the two proteins immobilized on the solid surfaces possessed dissociation constants KD. 10μM order to XynF3. We also analyzed interaction of esterase with another surfactant protein RolA attached to the two solid materials