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A novel method to identify nuclear proteins modified with N-acetylglucosamine by using a soluble glycosyltransferase having nuclear-localization signal

A novel method to identify nuclear proteins modified with N-acetylglucosamine by using a soluble glycosyltransferase having nuclear-localization signal
利用具有核定位信号的可溶性糖基转移酶鉴定 N-乙酰氨基葡萄糖修饰的核蛋白的新方法
批准号：
24659026
负责人：
YAMAMOTO Kazuo
金额：
$2.5万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2012
资助国家：
日本
项目状态：
已结题
起止时间：
2012-04-01 至 2014-03-31
来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659026/
关键词：
生化学糖鎖翻訳後修飾核内タンパク質

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中文摘要
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英文摘要
Glycosylation of proteins occurs in the lumens of the endoplasmic reticulum and the Golgi apparatus, which resulted in diverse structures of carbohydrate moieties. By contrast, addition of N-acetylglucosamine (GlcNAc) to serine and threonine is a unique glycosylation reaction occurred in nucleus and cytoplasm. We expressed a soluble glycosyltransferase having nuclear-localization signal in the cell and induced further elongation of GlcNAc residue. By using lectin affinity chromatography and mass spectrometry, we comprehensively identified O-GlcNAc modified proteins and their GlcNAc-modified amino acid residues.
期刊论文(2)
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科研奖励(0)
会议论文
Terminal N-acetylgalactosamine-specific leguminous lectin from Wisteria japonica as a probe for human lung squamous cell carcinoma.
DOI： 10.1371/journal.pone.0083886
发表时间： 2013
期刊： PloS one
影响因子： 3.7
作者： [Soga K, Teruya F, Tateno H, Hirabayashi J, Yamamoto K]
通讯作者： Yamamoto K
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  • 批准号：
    18K07235
  • 项目类别：
    Grant-in-Aid for Scientific Research (C)
  • 资助金额：
    $2.83万
  • 财政年份：
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  • 依托单位：
    Nagasaki University
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    26640082
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  • 资助金额：
    $2.5万
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Lightning to a wind turbine in a wind farm and its influence on damages
  • 批准号：
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  • 项目类别：
    Grant-in-Aid for Scientific Research (C)
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  • 财政年份：
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