Differentiation of plasmacytoid dendritic cells from iPS cells and its application for Vaccine development

本研究的目的是建立诱导多能干细胞（iPS）向浆细胞样树突状细胞分化的方法。GM-CSF和Flt 3L等细胞因子可有效促进iPS细胞来源的造血祖细胞向浆细胞样树突状细胞分化。此外，通过与0 P9饲养细胞共培养从人iPS细胞诱导人造血祖细胞。这些细胞可以表达作为造血标志物的CD 34和CD 43，这表明我们的方案可以适用于向人浆细胞样树突状细胞的分化。

The purpose of this study is to develop the differentiation protocols for plasmacytoid dendritic cells from induced pluripotent stem (iPS) cells. Cytokines, such as GM-CSF and Flt3L, could efficiently promote the differentiation into plasmacytoid dendritic cells from iPS cell-derived hematopoietic progenitor cells. Also, human hematopoietic progenitor cells were induced from human iPS cells by co-culture with OP9 feeder cells. These cells could express CD34 and CD43 as hematopoietic markers, suggesting that our protocols could apply to the differentiation into human plasmacytoid dendritic cells.