Analysis of human genes that enable high-efficiency gene targeting

分析人类基因，实现高效基因靶向

• 批准号: 24659035
• 负责人: ADACHI Noritaka
• 金额: $ 2.5万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659035/
• 关键词: ジーンターゲティング; FACS解析; ジーンターゲティンク

We have recently constructed an efficient system that enables rapid production of genetically modified human cell lines by gene targeting. Human cell mutants thus made will provide invaluable tools for human medicine and future drug development. In this research project, we have identified several human genes, which, when down-regulated, increase the efficiency of gene targeting by homologous recombination.

我们最近构建了一个有效的系统，可以通过基因靶向快速生产转基因人类细胞系。由此产生的人类细胞突变体将为人类医学和未来的药物开发提供宝贵的工具。在这个研究项目中，我们发现了几个人类基因，当这些基因下调时，通过同源重组提高了基因靶向的效率。

项目成果

Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs.

• DOI: 10.1084/jem.20111437
• 发表时间: 2012-05-07
• 期刊: The Journal of experimental medicine
• 作者: Malu S;De Ioannes P;Kozlov M;Greene M;Francis D;Hanna M;Pena J;Escalante CR;Kurosawa A;Erdjument-Bromage H;Tempst P;Adachi N;Vezzoni P;Villa A;Aggarwal AK;Cortes P
• 通讯作者: Cortes P

Repair of Accidental DNA Double-Strand Breaks in the Human Genome and Its Relevance to Vector DNA Integration

• DOI: 10.4172/2329-6682.1000e107
• 发表时间: 2014
• 作者: N. Adachi;Shinta Saito;Aya Kurosawa

DNA ligase IV and artemis act cooperatively to suppress homologous recombination in human cells: implications for DNA double-strand break repair.

• DOI: 10.1371/journal.pone.0072253
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kurosawa A;Saito S;So S;Hashimoto M;Iwabuchi K;Watabe H;Adachi N
• 通讯作者: Adachi N

C-terminal region of DNA ligase IV drives XRCC4/DNA ligase IV complex to chromatin

• DOI: 10.1016/j.bbrc.2013.08.068
• 发表时间: 2013-09-20
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Liu, Sicheng;Liu, Xunyue;Matsumoto, Yoshihisa
• 通讯作者: Matsumoto, Yoshihisa

Restoration of mismatch repair functions in human cell line Nalm-6, which has high efficiency for gene targeting.

• DOI: 10.1371/journal.pone.0061189
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Suzuki T;Ukai A;Honma M;Adachi N;Nohmi T
• 通讯作者: Nohmi T