Molecular mechanisms of prostaglandin elimination across brain barriers and its application to neural inflammatory diseases

免疫组织化学检测mPGES-1和L-PGDS蛋白的表达和定位。L-PGDS在薄脑膜细胞中表达。在小脑膜细胞和星形胶质细胞中检测到来自mPGES-1的信号。在小鼠炎症模型中，mPGES-1信号在形成血脑屏障（BBB）的脑毛细血管内皮细胞中被强烈检测到。在该模型中，参与炎症反应进展的PGD2的消除在血脑屏障中减弱。从这些结果来看，血脑屏障在调节前列腺素在大脑中的生理功能中起作用。

The expression and localization of mPGES-1 and L-PGDS proteins was examined by immunohistochemical analysis. L-PGDS were expressed in leptomeningeal cells. The signals derived from mPGES-1 were detected in leptomeningeal cells and astrocytes. In the inflammatory model of mice, this mPGES-1 signal was strongly detected in brain capillary endothelial cells, which forms the blood-brain barrier (BBB). In this model, the elimination of PGD2, which is involved in the progression of inflammatory responses, across the BBB was attenuated. From these results, the BBB plays a role in the regulation of the physiological function of prostaglandins in the brain.