Molecular mechanisms of prostaglandin elimination across brain barriers and its application to neural inflammatory diseases

前列腺素跨脑屏障消除的分子机制及其在神经炎症疾病中的应用

• 批准号: 24659072
• 负责人: HOSOYA Ken-ichi
• 金额: $ 2.5万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659072/
• 关键词: プロスタグランジン; 血液脳関門; 脳内炎症; プロスタグランジン合成酵素; 血液脳脊髄液関門; リポ多糖; 神経伝達物質; PGE2

The expression and localization of mPGES-1 and L-PGDS proteins was examined by immunohistochemical analysis. L-PGDS were expressed in leptomeningeal cells. The signals derived from mPGES-1 were detected in leptomeningeal cells and astrocytes. In the inflammatory model of mice, this mPGES-1 signal was strongly detected in brain capillary endothelial cells, which forms the blood-brain barrier (BBB). In this model, the elimination of PGD2, which is involved in the progression of inflammatory responses, across the BBB was attenuated. From these results, the BBB plays a role in the regulation of the physiological function of prostaglandins in the brain.

免疫组织化学检测mPGES-1和L-PGDS蛋白的表达和定位。L-PGDS在薄脑膜细胞中表达。在小脑膜细胞和星形胶质细胞中检测到来自mPGES-1的信号。在小鼠炎症模型中，mPGES-1信号在形成血脑屏障（BBB）的脑毛细血管内皮细胞中被强烈检测到。在该模型中，参与炎症反应进展的PGD2的消除在血脑屏障中减弱。从这些结果来看，血脑屏障在调节前列腺素在大脑中的生理功能中起作用。

项目成果

Role of the blood-cerebrospinal fluid barrier transporter as a cerebral clearance system for prostaglandin E2 produced in the brain.

血脑脊液屏障转运蛋白作为脑内产生的前列腺素 E2 的脑清除系统的作用。

• DOI: 10.1111/jnc.12018
• 发表时间: 2012
• 期刊: J Neurochem
• 影响因子: 4.7
• 作者: Eskilsson A.;Tachikawa M.;Hosoya K.;Blomqvist A.;Tachikawa M;Tachikawa M
• 通讯作者: Tachikawa M

Distribution of Microsomal Prostaglandin E Synthase-1 in the Mouse Brain

• DOI: 10.1002/cne.23593
• 发表时间: 2014-10-01
• 期刊: JOURNAL OF COMPARATIVE NEUROLOGY
• 影响因子: 2.5
• 作者: Eskilsson, Anna;Tachikawa, Masanori;Blomqvist, Anders
• 通讯作者: Blomqvist, Anders