Development of screening methods to find novel therapeutic drugs for neurodegenerative disease using fluorescence-based probe and high-content microscope.

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1. We developed novel fluorescence-based probe that can access the activity of chaperone-mediated autophagy (CMA) in a single cell level. Using this probe, we identified the decreased level of CMA in a cellular model of spinocerebellar ataxia type 14 (SCA14), indicating that CMA is a novel therapeutic target for neurodegenerative disease.2.Imaging analysis using SCA14 mutant gamma PKC,-GFP elucidated that HSP70, a member of molecular chaperone, is involved in the compensation of cytotoxicity induced by SCA14 mutant gamma PKC.