Investigating the role of IKK/NF-・B signaling axis on the cellular reprogramming

研究IKK/NF-・B信号轴对细胞重编程的作用

• 批准号: 24659133
• 负责人: KATO Tomohisa
• 金额: $ 2.41万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659133/
• 关键词: 細胞初期化; iPS 細胞; シグナル伝達; IKK; NF-κB

We have investigated the role, if any, of IKK/NF-κB signaling axis in cellular reprogramming into iPS cells. We found that ectopic expr ession of Yamanaka 4 factors induced the IKKβ kinase activity depending on ATM. Moreover, disruption of Ikkβ gene reduced the reprogramming efficiency, whereas co-expr ession of IKKβ^(EE), a constitutive active form of IKKβ, with Yamanaka 4 factors promoted the efficiency. We found disruption of Ikkβ elevated the number of senescent cells during the reprogramming. Collectively, our results indicate that IKKβ potentiates cellular reprogramming by antagonizing the cellular senescence evoked by the ectopic expression of Yamanaka factors in anAT M-dependent manner.

我们研究了IKK/NF-κ B信号轴在细胞重编程为iPS细胞中的作用。我们发现Yamanaka 4因子的异位表达依赖于ATM诱导IKK β激酶活性。此外，Ikk β基因的破坏降低了重编程效率，而IKK β ^（EE）（IKK β的组成性活性形式）与Yamanaka 4因子的共表达促进了重编程效率。我们发现Ikk β的破坏增加了重编程过程中衰老细胞的数量。总的来说，我们的研究结果表明，IKK β增强细胞重编程通过拮抗细胞衰老引起的异位表达Yamanaka因子在anATM依赖的方式。

项目成果

The transcription factor Sp3 regulates the expression of a metastasis-related marker of sarcoma, actin filament-associated protein 1-like 1 (AFAP1L1).

• DOI: 10.1371/journal.pone.0049709
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kajita Y;Kato T Jr;Tamaki S;Furu M;Takahashi R;Nagayama S;Aoyama T;Nishiyama H;Nakamura E;Katagiri T;Nakamura Y;Ogawa O;Toguchida J
• 通讯作者: Toguchida J

Cell context is important for SYT-SSX oncoprotein in epigenetic regulation of synovial sarcoma

细胞背景对于 SYT-SSX 癌蛋白在滑膜肉瘤表观遗传调控中很重要

• 发表时间: 2012
• 作者: Kajita Y;Kato T Jr;Tamaki S;Furu M;Takahashi R;Nagayama S;Aoyama T;Nishiyama H;Nakamura E;Katagiri T;Nakamura Y;Ogawa O;Toguchida J.;Kajiwara M;玉置さくら他
• 通讯作者: 玉置さくら他

Identification of interactors of fusion oncoprotein SYT-SSX delineates the mechanism underlying the epigenetic dysregulation in synovial sarcoma

融合癌蛋白 SYT-SSX 相互作用子的鉴定描绘了滑膜肉瘤表观遗传失调的机制

• 发表时间: 2012
• 作者: Kajita Y;Kato T Jr;Tamaki S;Furu M;Takahashi R;Nagayama S;Aoyama T;Nishiyama H;Nakamura E;Katagiri T;Nakamura Y;Ogawa O;Toguchida J.;Kajiwara M;玉置さくら他;加藤友久他
• 通讯作者: 加藤友久他

Cell context is an important factor for the role of SYT-SSX

细胞环境是 SYT-SSX 发挥作用的重要因素

• 发表时间: 2012
• 作者: Kajita Y;Kato T Jr;Tamaki S;Furu M;Takahashi R;Nagayama S;Aoyama T;Nishiyama H;Nakamura E;Katagiri T;Nakamura Y;Ogawa O;Toguchida J.;Kajiwara M;玉置さくら他;加藤友久他;Sakura Tamaki et al
• 通讯作者: Sakura Tamaki et al

滑膜肉腫の発生機構に関して

关于滑膜肉瘤发生的机制

• 发表时间: 2012
• 作者: Kajita Y;Kato T Jr;Tamaki S;Furu M;Takahashi R;Nagayama S;Aoyama T;Nishiyama H;Nakamura E;Katagiri T;Nakamura Y;Ogawa O;Toguchida J.;Kajiwara M;玉置さくら他;加藤友久他;Sakura Tamaki et al;玉置さくら他
• 通讯作者: 玉置さくら他